Human IL-23 ELISA Kit

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Cat: abs510013

Size: 96T

Human IL-23 ELISA Kit

Testing Principle

The high affinity plate in this kit is coated with a specific anti-human IL-6 antibody, utilizing the quantitative sandwich enzyme immunoassay technique. To initiate the assay, standards, test samples, and biotinylated detection antibodies are introduced into the wells of the plate labeled with enzymes. Following an incubation period, the IL-6 present in the samples forms immune complexes by binding to both the solid-phase antibodies and the detection antibodies. To remove any unbound substances, a thorough washing step is conducted. Next, horseradish peroxidase-labeled streptavidin (HRP) is introduced into the wells. After another round of washing, a chromogenic substrate is added, causing a color to develop in a light-protected environment. To halt the reaction, a stop solution is added, and the absorbance is subsequently measured at a wavelength of 450 nm, with reference correction wavelengths of either 540 nm or 570 nm.

Detection type: sandwich enzyme immunoassay

Form: pre-coated 96-well plate

Sample type: Cell culture supernatant, Serum, Plasma

Sample quantity: 100ul

Kit composition:

This kit includes a 96 well polystyrene microplate that has been coated with a capture antibody, along with a standard and a detection antibody for use in performing an ELISA assay. Additionally, the kit comes with a 10×reagent diluent for use in preparing sample and control solutions, as well as a Color reagent (A and B) for developing the assay signal. A 25×wash buffer is also included to aid in the removal of unbound material, along with a Stop solution for terminating the reaction. ELISA plate sealers are provided to aid in preventing evaporation of reaction solutions, and a comprehensive specification outlines the recommended assay protocol.

Sensitivity:  20.5 pg / mL

Detection range:  125 - 8000 pg/mL

Recovery rate:  93.9-103.2%

Storage: 2-8 ° C

The standard curve:

Background: IL-23

IL-23, a cytokine composed of the specific p19 and shared p40 subunits, is produced by activated macrophages, microglias and monocyte derived dendritic cells in response to pathogen attacks. This can include certain bacteria and viruses and/or their components. The presence of IL-23 is important for immune response and may be involved in the development of autoimmune diseases and cancer. Its role in these processes is still being studied.

The functional IL-23 receptor complex, composed of the IL-12 receptor β1 subunit and the specific IL-23 receptor subunit, is found in various immune cells such as Th1 and Th2 cells, bone marrow dendritic cells, activated macrophages, and memory T cells. This pathway plays a crucial role in initiating an immune response by recruiting the earliest neutrophils to the infection site. Additionally, IL-23 promotes the development and maintenance of Th17 cells, which are involved in autoimmune diseases and tumor progression. In humans, IL-23 is often up-regulated in immune disorders such as psoriasis, Crohn's disease, and multiple sclerosis. Overall, understanding the function of the IL-23 immune pathway can provide insights into potential treatments for these diseases.

Technical hints: For research use only, not for in vitro diagnosis.

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