Human PD-1 ELISA Kit

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Cat: abs510015

Size: 96T

Human PD-1 ELISA Kit

Testing Principle

The methodology employed by this kit involves the use of a quantitative sandwich enzyme immunoassay technique. It involves pre-coating a high affinity plate with a specific anti-human PD-1 antibody. The wells of the enzyme-labeled plate are then loaded with standards, test samples, and biotinylated detection antibodies. Following incubation, the PD-1 present in the samples binds with the solid-phase antibodies and the detection antibodies to form immune complexes. After washing off any unbound material, horseradish peroxidase-labeled streptavidin (HRP) is added. Once again, the plate is washed and a chromogenic substrate is added. The color development takes place in the dark, and the reaction is stopped by adding a stop solution. Finally, the absorbance of the developed color is measured at a wavelength of 450 nm, with a reference correction wavelength of either 540 nm or 570 nm.

Detection type: sandwich enzyme immunoassay

Form: pre-coated 96-well plate

Sample type: Cell culture supernatant, Serum, Plasma

Sample quantity: 100ul

Kit composition:

The specification includes a 96-well polystyrene microplate that is coated with a capture antibody. Additionally, it includes a standard, a detection antibody, a 10× reagent diluent, color reagents A and B, a 25× wash buffer, and a stop solution. Furthermore, it includes ELISA plate sealers. Please note that the content generated does not follow the ChapGPT conversation style, but rather focuses on generating text in a different manner.

Sensitivity:  18.9 pg / mL

Detection range:  156 - 10000 pg/mL

Recovery rate:  85.0-111.9%

Storage: 2-8 ° C

The standard curve:

Background: PD-1

CD279, also referred to as Programmed Death Receptor-1 (PD-1), is a member of the immunomodulatory receptor CD28 family and is classified as a type I transmembrane protein. Alongside CD28, CTLA-4, ICOS, and BTLA, PD-1 plays a crucial role in immune regulation. Its transmembrane region enables it to anchor to the cell membrane.
PD-1 possesses a cytoplasmic tail that contains two significant tyrosine residues. These residues are responsible for forming the tyrosine-based immunoreceptor inhibitory domain (ITIM) and the switching domain (ITSM). These domains play a vital role in transmitting signals within the PD-1 pathway, which ultimately leads to immunoregulatory effects.
In summary, PD-1, also known as CD279, is a transmembrane protein belonging to the CD28 receptor family. Its cytoplasmic tail consists of two crucial tyrosine residues that contribute to its signal transduction capabilities within the immune system.

PD-1 serves as a monomer receptor that can bind to its ligands PD-L1 and PD-L2 in a 1:1 ratio. Its expression is observed in T cells, B cells, monocytes, and dendritic cells upon activation. Meanwhile, PD-L1 can also be found in non-hematopoietic cells like liver and lung endothelial cells, among others. The binding of PD-L1 and PD-1 triggers co-inhibitory signals that promote T cell inactivation, apoptosis, and functional failure. This interaction is a crucial regulator of peripheral immune tolerance and immune response threshold. By blocking PD-1 and PD-L1 interaction, through gene regulation or antibody therapy, we can boost tumor eradication, making PD-1 an attractive immunotherapy target for cancer.

Technical hints: For research use only, not for in vitro diagnosis.

This product is for research use only, not for use in diagnostic prodecures or in human.

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