Mouse GM-CSF ELISA Kit

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Cat: abs520015

Size: 96T

Mouse GM-CSF ELISA Kit

Testing Principle

This enzyme immunoassay kit employs a quantitative sandwich technique, where a highly specific anti-mouse GM-CSF antibody is already coated on a plate with high binding affinity. The test samples, along with standards and biotinylated detection antibodies, are added to the wells of the enzyme-linked plate. The GM-CSF present in the samples interacts with the solid-phase antibodies, as well as those in the detection antibodies, forming immune complexes. Unbound material is then removed by washing, and horseradish peroxidase-labeled streptavidin (HRP) is added. In the next step, a chromogenic substrate is added to the mixture, and color development occurs in the dark. To stop the reaction, a stop solution is added, and absorbance is measured at a nominated wavelength of 450 nm (with reference correction wavelength of either 540 nm or 570 nm). Overall, the process is designed to allow for the quantitative measurement of GM-CSF in a given sample.

Detection type: sandwich enzyme immunoassay

Form: pre-coated 96-well plate

Sample type: Cell culture supernatant, Serum, Plasma

Sample quantity: 100ul

Kit composition:

This kit includes a 96 well polysyrene microplate that has been coated with a capture antibody and a detection antibody, along with a standard for reference. You'll also receive 10x reagent diluent to prepare your samples, color reagents (A and B) for detection, a 25x wash buffer, stop solution to halt the reaction, and ELISA plate sealers to ensure proper conditions. Please refer to the specification for more details and instructions on how to use this kit.

Sensitivity:  need to be improved

Detection range:   7.81-500 pg/mL

Recovery rate:    84-110%

Storage: 2-8 ° C

The standard curve:

Background:GM-CSF

GM-CSF, or CSF-2 for short, is a versatile cytokine belonging to the common β chain (βC) family of proteins. With a molecular weight of 30 kDa, GM-CSF adopts a α-helix conformation featuring a pair of in-chain disulfide bonds. This protein is primarily produced by immune cells, as well as various types of mesenchymal and epithelial cells. Secreted in the form of various glycosylated monomers, GM-CSF plays a crucial role in promoting the growth and maturation of a range of blood cells. Both IL-3 and IL-5 are also members of the βC family of cytokines.

GM-CSF, also known as granulocyte-macrophage colony-stimulating factor, plays a crucial role in inflammation by upregulating various cell types. These include encephalitis causing T cells, allergen-exposed lung endothelial cells, and IgE activated mast cells. In terms of structural similarity, the mature human GM-CSF shares 54% and 63% amino acid sequence identity with mouse and rat GM-CSF, respectively. The receptor for GM-CSF is composed of a 50 kDa ligand binding α subunit (GM-CSFRα) and a 120 kDa signal transduction βC subunit. Studies have reported that the functional GM-CSF receptor exists as a 2:2:2 complex of GM-CSF, GM-CSFRα, and βC. Additionally, the βC subunit is shared by the receptor complex of IL-3 and IL-5, indicating potential cross-signaling. Furthermore, GM-CSF can utilize Syndecan-2 as a co-receptor, expanding its signaling capabilities.

GM-CSF, also known as granulocyte-macrophage colony-stimulating factor, possesses various important functions. One of its primary abilities is to stimulate the production of monocytes, neutrophils, and eosinophils from CD34+ stem cell precursors. Additionally, GM-CSF acts together with IL-4 or FLT-3 ligand to support the development and maintenance of bone marrow and dermal dendritic cells. Moreover, it serves as a chemoattractant for neutrophils and dendritic cells, guiding them towards specific locations. Notably, GM-CSF plays a significant role in promoting Th1 and Th17 cell-mediated autoimmune inflammation, as well as inflammatory activation of dendritic cells, microglia, alveolar macrophages, and eosinophils. Furthermore, it collaborates with G-CSF, another type of colony-stimulating factor, to enhance tumor cell proliferation and invasion. These various features highlight the critical contributions of GM-CSF to immune responses, inflammation, and cellular development.

Technical hints: For research use only, not for in vitro diagnosis.

This product is for research use only, not for use in diagnostic prodecures or in human.

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