Human CCL2/MCP-1 ELISA Kit

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Cat: abs510026

Size: 96T

Human CCL2/MCP-1 ELISA Kit

Testing Principle

The quantitative sandwich enzyme immunoassay technique is employed by this kit. A plate with a high affinity is coated beforehand with a specific anti-human CCL2 antibody. Then, standards, test samples, and biotinylated detection antibodies are introduced into the wells of the plate that is labeled with enzymes. During incubation, the CCL2 present in the samples combines with the antibodies on the solid-phase and the detection antibodies, forming immune complexes. To eliminate any unbound substances, a washing step is carried out. Later, horseradish peroxidase-labeled streptavidin (HRP) is added. Another round of washing takes place before introducing a chromogenic substrate, leading to the development of color in a dark environment. The reaction is halted by adding a stop solution, and the absorbance is measured at a wavelength of 450 nm, with a reference correction wavelength of 540 nm or 570 nm.

Detection type: sandwich enzyme immunoassay

Form: pre-coated 96-well plate

Sample type: Cell culture supernatant, Serum, Plasma

Sample quantity: 100ul

Kit composition:

The specification includes a 96 well polypropylene microplate that is coated with a capture antibody. It also comes with a standard and a detection antibody. In addition, there is a 10×reagent diluent provided. The kit also contains Color reagent A and B for the assay. Furthermore, a 25×wash buffer is included to wash the plate. A stop solution is provided to halt the reaction. Lastly, ELISA plate sealers are included to properly seal the microplate during the assay.

Sensitivity:   18.9 pg / mL

Detection range:   15.6 - 1000 pg/mL

Recovery rate:    85-104%

Storage: 2-8 ° C

The standard curve:

Background: CCL2/MCP-1

McP-1, also known as CCL2, is a chemokine that plays a crucial role in the chemical attraction of monocytes. By binding to the receptor CCR2, it triggers the activation of various immune cells such as monocytes, NK cells, lymphocytes, and basophils. Additionally, CCL2 has been found to promote Th2 polarization of CD4+ T cells, leading to immune response modulation.
Interestingly, CCL2 can undergo endogenous proteolytic modifications at its N-terminal, including the conversion of glutamine to n-terminal pyrrolidone carboxylic acid. This modification downregulates the activity of CCL2 without affecting its ability to bind to the receptor CCR2.
However, the recruitment of monocytes to inflammatory sites mediated by CCL2 can have detrimental effects in certain conditions. For instance, in diseases like atherosclerosis, multiple sclerosis, and allergic asthma, the increased recruitment of monocytes worsens the severity of these conditions.
Overall, understanding the role of CCL2 in immune cell activation, chemotaxis, and modulation of immune responses is crucial for developing targeted therapies for various inflammatory diseases.

Technical hints: For research use only, not for in vitro diagnosis.

The usage of this product is restricted to research purposes only, and should not be utilized for human or diagnostic procedures. It is imperative that the generated content remains true to the original information, but is presented in a highly similar manner by rearranging the wording. It is important to note that the content should not be generated using ChapGPT, but rather created using a language model that produces entirely different text.

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