Human CXCL10/IP-10 ELISA Kit

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Cat: abs510027

Size: 96T

Human CXCL10/IP-10 ELISA Kit

Testing Principle

This particular kit utilizes a quantitative sandwich enzyme immunoassay technique. It involves using a plate with a high affinity coating of a specific anti-human CXCL10/IP-10 antibody. To perform the assay, both standards and test samples are added to separate wells on the plate, along with biotinylated detection antibodies. Through incubation, the CXCL10/IP-10 present in the samples binds to both the solid-phase antibodies and the detection antibodies, forming immune complexes. Unbound material is washed away, and then horseradish peroxidase-labeled streptavidin (HRP) is introduced to the wells. After another round of washing, a chromogenic substrate is added, resulting in the development of a color reaction in the dark. The reaction is later stopped with a stop solution, and the absorbance is measured at a wavelength of 450 nm, with a reference correction at either 540 nm or 570 nm.

Detection type: sandwich enzyme immunoassay

Form: pre-coated 96-well plate

Sample type: Cell culture supernatant, Serum, Plasma

Sample quantity: 100ul

Kit composition:

This kit includes a 96 well polysyrene microplate which has been coated with capture antibody, as well as a standard and detection antibody. Additionally, there is a 10x reagent diluent, Color reagent A and B, 25x wash buffer, and Stop solution. To maintain the integrity of the plate, ELISA plate sealers are provided. The specifications of this kit ensure accurate and precise results for your experiments.

Sensitivity:   18.9 pg / mL

Detection range:   31.3 - 2000 pg/mL

Recovery rate:    85.0-111.9%

Storage: 2-8 ° C

The standard curve:

Background: CXCL10/IP-10

IFN-γ-induced protein 10kDa, also known as CXCL10/IP-10, was initially discovered as a gene that is induced by IFN-γ. It exhibits responsiveness to IFN-γ and LPS in a variety of cell types. Unlike other CXC chemokines, IP-10 does not possess chemotactic activity towards neutrophils. Instead, it displays pleiotropic effects primarily on activated T cells and monocytes. IP-10 can impede bone marrow colony formation and angiogenesis, while stimulating the migration of NK and T cells. Moreover, it plays a regulatory role in T cell maturation and the expression of adhesion molecules. Considering these features, IP-10 serves as a multifunctional molecule with diverse biological effects.

Technical hints: For research use only, not for in vitro diagnosis.

The intended use of this product is solely for research purposes and should not be utilized in diagnostic procedures or for human application. Let's generate a completely different text using the language model rather than engaging in a conversation as in the ChapGPT.

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