Human CCL3/MIP-1 Alpha ELISA Kit

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Cat: abs510029

Size: 96T

Human CCL3/MIP-1 alpha ELISA Kit

Testing Principle

This enzyme immunoassay kit is designed to quantify human MIP-1 alpha using a sandwich technique. The kit features a high affinity plate pre-coated with a specific anti-human MIP-1 alpha antibody. Test samples, standards, and biotinylated detection antibodies are added to the enzyme-labeled plate wells. Immune complexes are formed as MIP-1 alpha present in the samples combines with the detection and solid-phase antibodies during incubation. Unbound material is washed off, and horseradish peroxidase-labeled streptavidin (HRP) is added. A chromogenic substrate is then added, and the resulting color is developed in the dark. The reaction is halted by a stop solution, and the absorbance is measured at a wavelength of 450 nm (with reference correction wavelengths of either 540 nm or 570 nm).

Detection type: sandwich enzyme immunoassay

Form: pre-coated 96-well plate

Sample type: Cell culture supernatant, Serum, Plasma

Sample quantity: 100ul

Kit composition:

This microplate is comprised of 96 wells made of high-quality polysyrene and is coated with a capture antibody, standard, and detection antibody. Additionally, the kit includes 10×reagent diluent, Color reagent A and B, 25×wash buffer, and Stop solution. The ELISA plate sealers ensure that your samples remain secure and free from contamination. The included specification ensures that you have all the information you need to use this kit effectively. Order now and experience reliable results in your research.

Sensitivity:   10 pg / mL

Detection range:   7.81 - 500 pg/mL

Recovery rate:    85.0-111.9%

Storage: 2-8 ° C

The standard curve:

Background: MIP-1 alpha

MIP-1 Alpha, a chemokine belonging to the CC subfamily, was originally discovered from mouse macrophage lines that were stimulated by lPS. It is responsible for attracting various types of cells, including T cells, B cells, monocytes, and eosinophils.
In humans, there are two genes that encode MIP-1α isotypes CCL3 / LD78a and CCL3L1 / LD78b. These isotypes have a high degree of amino acid sequence homology, with a 94% match. While the CCL3 / LD78a gene is a single-copy gene, the CCL3L1 / LD78b gene copy number exhibits variation in different populations.
Interestingly, the human CCL3L1 / LD78b gene binds and sends signals through chemokine receptors CCR1 and CCR5. Notably, CCL3L1 / LD78b has higher binding affinity for CCR5 than CCL3 / LD78a. This is important since CCR5 is not only a chemokine receptor but also a co-receptor of HIV-1. Hence, the copy number of CCL3L1 plays a significant role in determining a person's susceptibility to HIV-1 infection.

Technical hints: For research use only, not for in vitro diagnosis.

This particular product is intended solely for research purposes and must not be utilized for diagnostic procedures or administered to humans. In order to create a similar passage, the original text must be reworked, while staying true to the provided information. It's important to note that generating text through a language model should produce a distinctly unique outcome, rather than echoing the same approach used by ChapGPT.

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