Human IL-27 ELISA Kit

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Cat: abs510021

Size: 96T

Human IL-27 ELISA Kit

Testing Principle

The quantitative sandwich enzyme immunoassay technique is utilized in this kit. The plate has a high affinity and is pre-coated with a specific anti-human IL-27 antibody. To conduct the assay, standards, test samples, and biotinylated detection antibodies are added to the wells of the enzyme-labeled plate. Subsequently, incubation takes place, allowing the IL-27 in the samples to combine with the solid-phase antibodies and detection antibodies, forming immune complexes. After unbound material is removed through washing, horseradish peroxidase-labeled streptavidin (HRP) is introduced. Further washing is performed, followed by the addition of a chromogenic substrate. In the absence of light, the color develops. The reaction is stopped using a stop solution, and the absorbance is then measured at a wavelength of 450 nm. It is important to note that a wavelength of 540 nm or 570 nm is used for reference correction. By ensuring the generated content is based on the original text information, a highly similar content can be generated.

Detection type: sandwich enzyme immunoassay

Form: pre-coated 96-well plate

Sample type: Cell culture supernatant, Serum, Plasma

Sample quantity: 100ul

Kit composition:

This kit includes a 96 well polystyrene microplate that has been coated with a capture antibody, as well as a standard and detection antibody. Additionally, the kit includes a 10×reagent diluent, color reagent (both A and B), and a 25×wash buffer. Furthermore, a stop solution is provided, along with ELISA plate sealers, and a specification document. Overall, this kit provides essential components for conducting an ELISA assay with ease and accuracy.

Sensitivity:  1.8 pg / mL

Detection range:   156 - 10000 pg/mL

Recovery rate:  82-110%

Storage: 2-8 ° C

The standard curve:

Background: IL27

IL-27, a cytokine composed of EBI3 and p28 subunits, functions as a heterodimer. EBI3 is associated with the p40 subunit of IL-12 and IL-23, while p28 is associated with the p35 chain of IL-12. This cytokine is expressed by various cells including monocytes, endothelial cells, and dendritic cells. It acts through a receptor complex consisting of IL-27 R alpha/WSX-1/TCCR and GP130.
IL-27 plays a role in sensitizing naive CD4+ T cells to IL-12-induced Th1 development. By inducing the expression of IL-12 receptors on these cells, IL-27 makes them more responsive to the effects of IL-12. Additionally, IL-27 acts as an anti-inflammatory cytokine by restricting the activity of T cells and NKT cells. This regulatory function helps to limit the inflammatory response and maintain immune homeostasis.

Technical hints: For research use only, not for in vitro diagnosis.

This specific product is strictly intended for research purposes only and should not be utilized for any diagnostic procedures or administered to humans. It is imperative that users abide by these guidelines to ensure product safety and to maintain ethical research practices. Please be aware of the potential repercussions that may result from noncompliance with these regulations.

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