Human IL-6 ELISA Kit

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Cat: abs510003

Size: 96T

Human IL-6 ELISA Kit

Testing Principle

The diagnostic kit provided applies a quantitative sandwich enzyme immunoassay methodology. The plate is pre-coated with a specific anti-human IL-6 antibody with high affinity. The wells of the enzyme-labeled plate receive standards, test samples, and biotinylated detection antibodies. During incubation, the IL-6 present in the samples come together with the solid-phase antibodies and the detection antibodies to create immune complexes. Following a wash to eliminate any unbound material, horseradish peroxidase-labeled streptavidin (HRP) was introduced. A chromogenic substrate was added subsequently, and the color developed under dark conditions. After adding a stop solution to halt the reaction, the absorbance measurement occurred at a wavelength of 450 nm, with the reference correction wavelength of 540 nm or 570 nm.

Detection type: sandwich enzyme immunoassay

Form: pre-coated 96-well plate

Sample type: Cell culture supernatant, Serum, Plasma

Sample quantity: 100ul

Kit composition:

The kit includes a 96 well microplate made of polysyrene and coated with a capture antibody. It also includes a standard, a detection antibody, and a 10× reagent diluent. You will also receive color reagents A and B, a 25× wash buffer, and a stop solution. In addition, ELISA plate sealers are included to ensure accuracy during testing. The kit's specifications are outlined for your convenience.

Sensitivity:1.56 pg / mL

Detection range:  9.38 - 600 pg/mL

Recovery rate: 94-111%

Storage: 2-8 ° C

The standard curve:

Background: IL-6

IL-6 is a cytokine that plays multiple roles in various physiological processes, including inflammation, hematopoiesis, bone metabolism, and cancer progression. It is a phosphorylated and glycosylated a-helical protein that ranges in weight from 22-28 kDa. The mature human IL-6 consists of 183 amino acids and shares 39% aa sequence identity with mouse and rat IL-6. Alternative splicing generates several isoforms, and some of them exhibit antagonistic properties.
IL-6 is expressed by various cells types, including CD8+ T cells, fibroblasts, synoviocytes, and adipocytes, among others. Its production is correlated with cell activation and is controlled by hormones like glucocorticoids, catecholamines, and secondary sex steroids. IL-6 levels in normal human circulating range at about 1 pg/mL, which is slightly elevated during the menstrual cycle and modestly elevated in certain cancers. Large elevations in circulating IL-6 occur after surgery.
Besides the cell types mentioned earlier, some other cells that express IL-6 include osteoblasts, megakaryocytes, endothelial cells, cerebral cortex neurons, adrenal medulla chromaffin cells, retinal pigment cells, mast cells, keratinocytes, Langerhans cells, fetal and adult astrocytes, neutrophils, monocytes, eosinophils, colonic epithelial cells, B1 B cells and pancreatic islet beta cells. This underscores the diverse range of physiological functions that IL-6 is capable of mediating in the body.

IL-6 induces signaling through a cell surface heterodimeric receptor complex composed of a ligand binding subunit (IL-6 R alpha) and a signal transducing subunit (gp130). IL-6 binds to IL-6 Ra, triggering IL-6 Ra association with gp130 and gp130 dimerization. gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM. Soluble forms of IL-6 Ra are generated by both alternative splicing and proteolytic cleavage. In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 Ra elicit responses from gp130-expressing cells that lack cell surface IL-6 Ra. Trans-signaling enables a wider range of cell types to respond to IL-6, as the expression of gp130 is ubiquitous, while that of IL-6 Ra is predominantly restricted to hepatocytes, monocytes, and resting lymphocytes. Soluble splice forms of gp130 block trans-signaling from IL-6/IL-6 Ra but not from other cytokines that use gp130 as a co-receptor.

IL-6, along with TNF-a and IL-1, drives the acute inflammatory response. IL-6 is almost solely responsible for fever and the acute phase response in the liver, and it is important in the transition from acute inflammation to either acquired immunity or chronic inflammatory disease. When dysregulated, it contributes to chronic inflammation in conditions such as obesity, insulin resistance, inflammatory bowel disease, arthritis, and sepsis. IL-6 modulates bone resorption and is a major effector of inflammatory joint destruction in rheumatoid arthritis through its promotion of Th17 cell development and activity. It contributes to atherosclerotic plaque development and destabilization as well as the development of inflammation-associated carcinogenesis.

Technical hints: For research use only, not for in vitro diagnosis.

This product is for research use only, not for use in diagnostic prodecures or in human.

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