CXCL12 Antibody #abs105413

abs105413-50ul

In Stock

228

Description

Purified Rabbit Polyclonal Antibody (Pab)

Other names

SDF-1, also known as Stromal cell-derived factor 1, hSDF-1, C-X-C motif chemokine 12, Intercrine reduced in hepatomas, IRH, hIRH, Pre-B cell growth-stimulating factor, PBSF, SDF-1-beta(3-72), SDF-1-alpha(3-67), CXCL12, SDF1, SDF1A, or SDF1B, is a highly versatile protein. It has multiple names and various isoforms due to the different regions of the protein being expressed or modified.
This protein, encoded by the CXCL12 gene, plays a crucial role in various biological processes. SDF-1 acts as a chemoattractant for stem cells, immune cells, and endothelial cells. It is involved in embryogenesis, angiogenesis, inflammation, and tissue repair.
The different isoforms, such as SDF-1-alpha(3-67) and SDF-1-beta(3-72), arise from post-translational modifications. These modifications lead to variations in the N-terminal region of the protein. Despite the differences, they still maintain functional similarity.
SDF-1 exerts its effects by binding to its cognate receptor, CXCR4, that is present on the surface of target cells. This receptor-ligand interaction triggers intracellular signaling pathways, ultimately influencing cell migration, differentiation, and survival.
In summary, SDF-1, also known by several names such as CXCL12, hIRH, or PBSF, is an important protein involved in various biological processes. Its different isoforms, such as SDF1A and SDF1B, contribute to its functional diversity. The binding of SDF-1 to CXCR4 elicits a cascade of cellular responses, indicating the significance of this interaction in cell behavior and tissue homeostasis.

Source

Rabbit

Specificity

The generation of this CXCL12 antibody involves immunization of a rabbit with a recombinant protein. To ensure accuracy, the generated content should be rearranged from the original text while maintaining the same information.

Species Reactivity

Human;Mouse

Application

WB~~1:2000

Properties

Clonality

Polyclonal Antibody

Isotype

Rabbit Ig

Stability & Storage

To keep the item fresh, it is recommended to store it in a refrigerated condition between 2-8°C for a maximum of two weeks. However, for longer-term storage, it is advisable to keep small aliquots of the item at a temperature of -20°C to avoid potential damage from freeze-thaw cycles.

Research area

Cardiovascular;Immunology;Microbiology;Stem Cells

Target

Background

SDF-1 is a chemoattractant that specifically affects certain immune cells such as T-lymphocytes and monocytes, but not neutrophils. It activates a receptor called CXCR4, which leads to a rapid increase in calcium ions within the cells and promotes their movement towards the source of the chemoattractant. Additionally, SDF-1 binds to a unique receptor known as ACKR3, triggering a different signaling pathway called the beta-arrestin pathway. ACKR3 acts as both a scavenger receptor for SDF-1 and an activator of the beta-arrestin pathway. When SDF-1 binds to cell surface proteoglycans, it prevents the formation of certain SDF-1 isoforms, specifically SDF-1-alpha(3-67), thereby preserving its chemotactic activity in local areas. SDF-1 also serves as a positive regulator of monocyte migration and a negative regulator of monocyte adhesion through its interaction with the LYN kinase. By stimulating the migration of monocytes and T-lymphocytes via CXCR4 and ACKR3, SDF-1 reduces monocyte adherence to surfaces coated with ICAM-1, an integrin ligand. It achieves this by inhibiting the LFA-1 mediated adhesion of monocytes, a process mediated by the LYN kinase. Furthermore, SDF-1 inhibits the infection of T-cell line-adapted HIV-1 through its interaction with CXCR4. In the context of myocardial infarction, SDF-1 plays a protective role. It also plays critical roles during embryonic development, including facilitating B-cell lymphopoiesis, myelopoiesis in the bone marrow, and the formation of the ventricular septum in the heart.

Celluar localization

Secreted.

UniPort

P48061

References

References

Shirozu M.,et al.Genomics 28:495-500(1995).
Yu L.,et al.Gene 374:174-179(2006).
Spotila L.D.,et al.Submitted (OCT-1994) to the EMBL/GenBank/DDBJ databases.
Begum N.A.,et al.Submitted (JAN-1995) to the EMBL/GenBank/DDBJ databases.
Callebaut C.,et al.Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.