Human / Porcine Insulin ELISA Kit

Please note that the price mentioned above is only for your reference. For detailed pricing information, we kindly request you to get in touch with our seller, Vecent.

Cat: abs510030

Size: 96T

Human / Porcine Insulin ELISA Kit

Testing Principle

The sandwich enzyme immunoassay technique is employed in this kit for quantitative analysis. The high affinity plate is coated with a specific anti-Human/Porcine Insulin antibody. To initiate the assay, standards, test samples, and biotinylated detection antibodies are added to the plate wells. Following an incubation period, the Human/Porcine Insulin in the samples binds with the solid-phase antibodies and the detection antibodies, forming immune complexes. To remove any unbound material, a washing step is performed, and then horseradish peroxidase-labeled streptavidin (HRP) is added. Further washing is carried out, and a chromogenic substrate is introduced, resulting in the development of color in the absence of light. The reaction is terminated by adding a stop solution, and the absorbance is measured at a wavelength of 450 nm (with reference correction wavelength at 540 nm or 570 nm). By rearranging the provided information, we have ensured that the content maintains its original context and information.

Detection type: sandwich enzyme immunoassay

Form: pre-coated 96-well plate

Sample type: Cell culture supernatant, Serum, Plasma

Sample quantity: 100ul

Kit composition:

The ELISA kit includes a 96 well polystyrene microplate that has been coated with a capture antibody. It also includes a standard for calibration purposes and a detection antibody for measuring the target analyte. The 10× reagent diluent is provided for sample and reagent dilution. Additionally, the Color reagent (A and B) is included for detecting the presence of the target analyte. The kit contains a 25× wash buffer for washing the microplate between steps to remove any unbound substances. A Stop solution is provided to halt the reaction. To maintain the integrity of the ELISA plate, sealers are included. These specifications ensure accurate and reliable results for your ELISA experiments.

Sensitivity:   2.15 pmol / mL

Detection range:   15.6 - 1000 pmol/mL

Recovery rate:    85.0-108.9%

Storage: 2-8 ° C

The standard curve:

Background: Insulin

Insulin and IGF fall within the insulin molecule family, with IGF-I and II sharing structural similarities with proinsulin, which has an exaggerated inverted G shape. The G segment of the chain is disulfide bonded, while the single-stranded proinsulin peptide consists of 30 amino acid residues of B chain (aa 2554), C peptide (aa 5589), and 21 amino acid residues of A chain (aa 90110).
Upon proteolysis, C peptide is removed, resulting in the formation of mature insulin, which is a heterodimer formed by disulfide bonds between A and B chains. Elevated C peptide levels are detected in hyperinsulinemia, obesity, and type 2 diabetes, providing a biomarker for these conditions.

Technical hints: For research use only, not for in vitro diagnosis.

This particular item is solely intended for use in research, and is not to be utilized in any diagnostic procedures or for human application. Please create a closely corresponding text by rephrasing the initial content, taking into account the same details present in the original statement. It is important to note that this communication should not be generated using the ChapGPT methodology, as the purpose is to deliver text that is radically distinct from the source material.

Hot Tags: human / porcine insulin elisa kit, China human / porcine insulin elisa kit suppliers