Mouse IL-4 ELISA Kit

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Cat: abs520003

Size: 96T

Mouse IL-4 ELISA Kit

Testing Principle

The Mouse IL-4 ELISA Kit employs a quantitative sandwich enzyme immunoassay technique. Initially, a high affinity plate is coated with a specific anti-mouse IL-4 antibody. Next, the plate wells are filled with standards, test samples, and biotinylated detection antibodies. These components then interact with the solid-phase antibodies, forming immune complexes. To remove any unbound material, a thorough washing is performed. Subsequently, horseradish peroxidase-labeled streptavidin (HRP) is added to the plate. After another round of washing, a chromogenic substrate is introduced, resulting in color development in the absence of light. To halt the reaction, a stop solution is added. Lastly, the absorbance is measured at a wavelength of 450 nm, with a reference correction wavelength of 540 nm or 570 nm. By rearranging the provided information, a highly similar content can be generated.

Detection type: sandwich enzyme immunoassay

Form: pre-coated 96-well plate

Sample type: Cell culture supernatant, Serum, Plasma

Sample quantity: 100ul

Kit composition:

The specifications for the 96-well polystyrene microplate include a coating of capture antibody. Additionally, the microplate comes with a standard, a detection antibody, a 10x reagent diluent, and color reagents A and B. The package also includes a 25x wash buffer, stop solution, and ELISA plate sealers. Please note that the content generated has been rearranged to reflect the original information provided.

Sensitivity:  2.3 pg/mL

Detection range:   15.6 - 1,000 pg/mL

Recovery rate:  75-112%

Storage: 2-8 ° C

The standard curve:

Background: IL-4

The Mouse IL-4 ELISA Kit detects a secretory protein consisting of 129 amino acids. IL-4 is mainly produced by activated T lymphocytes, mast cells, and basophils. It plays a crucial role in regulating various immune responses by acting on different cell types. IL-4 has significant involvement in B cell activation processes and triggers the expression of class II MHC molecules in dormant B cells. Additionally, IL-4 acts as a common stimulant in DNA synthesis. It has the ability to enhance the secretion and cell surface expression of IgE and IgG1 antibodies. Furthermore, IL-4 controls the expression of low affinity Fc receptors for IgE (CD23) on lymphocytes and monocytes. An interesting function of IL-4 is its positive regulation of IL31RA expression in macrophages. By rearranging the provided information, a highly similar content can be formed while maintaining the original text's context and details.

Technical hints: For research use only, not for in vitro diagnosis.

The intended purpose of this product is solely for research use and it is explicitly not intended for utilization in diagnostic procedures or for human application. To reiterate, this product should not be utilized for diagnostic purposes or in any way involving human subjects.

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