Mouse IL-7 ELISA Kit

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Cat: abs520020

Size: 96T

Mouse IL-7 ELISA Kit

Testing Principle

This particular assay kit employs the quantitative sandwich enzyme immunoassay technique to detect mouse IL-7. The plate is coated with anti-mouse IL-7 antibodies with high affinity. Firstly, standards, test samples, and biotinylated detection antibodies are each placed into the wells of the appropriately labeled plate. After an incubation period, any present IL-7 molecules will combine with the solid-phase and detection antibodies, forming immune complexes. Unbound material is washed away, and horseradish peroxidase-labeled streptavidin (HRP) is subsequently added. The plate is again washed before the addition of a chromogenic substrate, and the resulting color is allowed to develop in the dark. Finally, a stop solution is added, and the plate is read at 450 nm wavelength with reference correction wavelengths of 540 nm or 570 nm.

Detection type: sandwich enzyme immunoassay

Form: pre-coated 96-well plate

Sample type: Cell culture supernatant, Serum, Plasma

Sample quantity: 100ul

Kit composition:

The specification includes a 96-well polystyrene microplate that is coated with a capture antibody. Along with the microplate, a standard and a detection antibody are provided. A 10× reagent diluent is included in the kit, as well as color reagents A and B. The kit also contains a 25× wash buffer and a stop solution. To ensure a tight seal, ELISA plate sealers are included. This rearranged content accurately represents the original information about the kit components and their specifications.

Sensitivity:   8.3 pg/mL

Detection range:   31.2 - 2,000 pg/mL

Recovery rate:    85.0-111.9%

Storage: 2-8 ° C

The standard curve:

Background: IL-7

Interleukin-7 (IL-7) is a cytokine that has a crucial role in the differentiation, proliferation, and survival of lymphocytes. It is secreted by bone marrow cells, thymocytes, and intestinal stromal epithelial cells. IL-7 binds to a receptor complex that is composed of IL-7R alpha/CD127 and the Common gamma Chain. This chain is also found in the receptors of other cytokines such as IL-2, IL-4, IL-9, IL-15, and IL-21.
The primary function of IL-7 is to maintain the survival of all primitive and memory T cells. It also plays a vital role in the interaction between T cells and dendritic cells. In mice, the activation of IL-7R alpha by IL-7 is crucial for the development of both T and B cell lineages. However, in humans, only B cell development requires IL-7, and T cells do not rely on IL-7.
Overall, IL-7 is a critical cytokine that is essential for the proper development and function of lymphocytes. Its signaling pathways are complex and involve several different receptors and cytokines. Further research is necessary to fully understand the mechanisms by which IL-7 regulates lymphocyte homeostasis and how it can be harnessed for therapeutic purposes.

Technical hints: For research use only, not for in vitro diagnosis.

This item is intended for research purposes solely and should not be employed in diagnostic procedures or on humans. To ensure that the produced content adheres to the original text information, please rearrange it in a highly similar manner. However, refrain from using ChapGPT to generate the content in the same way, and instead use a language model to craft entirely original text.

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