Mouse IL-17 ELISA Kit

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Cat: abs520009

Size: 96T

Mouse IL-17 ELISA Kit

Testing Principle

In this kit, the sandwich enzyme immunoassay technique is employed for quantitative analysis. Initially, a plate with a high affinity is coated with a specific anti-mouse IL-17/IL-17A antibody. Then, the wells of the plate are filled with standards, test samples, and biotinylated detection antibodies. The IL-17/IL-17A present in the samples binds with the solid-phase antibodies and the detection antibodies, forming immune complexes. Unbound materials are removed through a washing process. Next, horseradish peroxidase-labeled streptavidin (HRP) is introduced. After washing again, a chromogenic substrate is added, and the color develops in darkness. To halt the reaction, a stop solution is added, and the absorbance is measured at a wavelength of 450 nm (reference correction wavelength of 540 nm or 570 nm).

Detection type: sandwich enzyme immunoassay

Form: pre-coated 96-well plate

Sample type: Cell culture supernatant, Serum, Plasma

Sample quantity: 100ul

Kit composition:

The specification includes a 96 well polystyrene microplate coated with capture antibody, a standard, a detection antibody, a 10× reagent diluent, Color reagent A and B, a 25× wash buffer, a Stop solution, and ELISA plate sealers.

Sensitivity:  1.8 pg / mL

Detection range:   15.6 - 1000 pg/mL

Recovery rate:  82-110%

Storage: 2-8 ° C

The standard curve:

Background: IL-17

The IL-17 family encompasses several pro-inflammatory cytokines sharing a conserved cysteine junction structure but differing at the N-terminal. These glycoproteins are secreted as dimers and exert their effects by stimulating local cytokine production and recruiting granulocytes to sites of inflammation. IL-17 is primarily induced by IL-17 and IL-23 in activated CD4+ T cells, setting it apart from Th1 or Th2 cells. On the other hand, IL-17f shows the closest resemblance to IL-17 but is only triggered by IL-23 in activated monocytes. IL-17b binds to the IL-17B receptor and lacks affinity for the IL-17 receptor. It shares the highest homology with IL-17D, which is expressed by resting CD4+ T cells and CD19+ B cells. Th2 cells predominantly produce IL-17e, which recruits eosinophils to lung tissue. Lastly, IL-17c exhibits a restricted expression pattern, with known detection in the adult prostate and fetal kidney Banks. By rearranging and summarizing the given information, we can understand the various members of the IL-17 family and their roles in inflammation and immune response.

Technical hints: For research use only, not for in vitro diagnosis.

The intended purpose of this product is solely for research applications and it should not be employed for human use or diagnostic procedures. Please create content that closely aligns with the original text while rearranging the words, so that it conveys the same message. Additionally, refrain from generating content using ChapGPT and instead use a different language model approach to produce distinct text.

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