Mouse CXCL1/KC (IL-8) ELISA Kit

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Principle of Detection: The double antibody sandwich ELISA method is employed in this experiment. The microtiter plate is coated with the anti-mouse CXCL1/KC monoclonal antibody. The antibody immobilized on the plate will bind with the CXCL1/KC present in the sample and standard, followed by washing away of the unbound components. Then, the horseradish peroxidase-labeled anti-mouse CXCL1/KC polyclonal antibody is added, and any unbound antibody is washed away. Subsequently, the substrate solution (color developer) is added, and the color of the solution correlates with the amount of bound target protein. Finally, the absorbance is measured using a microplate reader.
Type of Test: Double antibody sandwich method
Format: Pre-coated 96-well plate
Sample Types: Cell supernatant, serum, plasma
Sample Volume: 100ul
Kit Components: Pre-coated 96-well plate, standard, anti-mouse CXCL1/KC detection antibody, dilution buffer, color developing solution (A, B), washing solution, stop solution, SA-HRP, sealing film, and a copy of instructions.
Sensitivity: Refer to instructions
Detection Range: 15.6-1000 pg/mL
Recovery Rate Range: 84-110%
Storage Method: Store at 2-8°C
Background:
CXCL1 (GRO alpha) is a member of the CXC chemokine family and contains the ELR motif, which selectively attracts neutrophils. Human CXCL1 undergoes post-translational modification and is divided into three isoforms: CXCL1 (4-73), CXCL1 (5-73), and CXCL1 (6-73). These isoforms are produced by proteolytic cleavage after secretion by peripheral blood mononuclear cells, exhibiting higher in vitro activity than the full-length protein. CXCL1 has high sequence homology with two other CXC proteins, CXCL2 (or GRO beta) and CXCL3 (or GROγ), with 90% and 86% amino acid sequence identity, respectively. Human CXCL1 mRNA is expressed in various cells, including foreskin fibroblasts, synovial fibroblasts, chondrocytes, bone cells, breast fibroblasts, epithelial cells, endothelial cells, activated monocytes, macrophages, and neutrophils. CXCL1 is constitutively overexpressed in tumorigenic cells, resulting in elevated levels of CXCL1 in different types of tumors and cancers like lung cancer and melanoma. CXCL1 is a member of the CXC chemokine family, which plays a role in diverse biological processes such as allergic reactions, angiogenesis, inflammation, tumor growth, and metastasis. In mice, CXCL2 and CXCL3 share 67% and 60% sequence homology with CXCL1, respectively. Furthermore, rat CXCL1 and mouse CXCL1 exhibit 89% amino acid sequence identity. Homologs of IL-8 have been identified in species like sheep, rabbits, pigs, and dogs, but there is currently no clear definition for IL-8 homologs in mice and rats. However, MIP-2 (CCL8) and KC (CXCL1) are widely regarded as functional homologs of IL-8 protein in murine species.

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