Mouse CXCL2/MIP-2 ELISA Kit

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Principle of Detection: The detection principle utilized in this kit involves the double antibody sandwich enzyme-linked immunosorbent detection technology. To begin with, a highly specific anti-mouse CXCL2/MIP-2 antibody is pre-coated on an ELISA plate that exhibits high affinity. The plate is comprised of 96 wells. In order to detect CXCL2/MIP-2, the standard substance, along with the sample being tested and the biotinylated detection antibody, are added to the wells of the enzyme-labeled plate. Following an incubation period, the CXCL2/MIP-2 present in the sample binds to both the solid phase antibody and the detection antibody, forming an immune complex. The subsequent step involves washing the plate to remove any unbound substances, after which, horseradish peroxidase-labeled streptavidin (Streptavidin-HRP) is added. Another wash cycle follows, then a chromogenic substrate is added to induce color development. The reaction is halted by adding a stop solution, and finally, the absorbance value is measured at a wavelength of 450 nm, with reference correction wavelengths at 540nm or 570nm.
Test Type: The test is conducted using the double antibody sandwich method.
Form: The ELISA plate comes pre-coated with the specific anti-mouse CXCL2/MIP-2 antibody.
Test Sample Type: The kit is designed for testing cell supernatant, serum, and plasma samples.
Sample Volume: A sample volume of 100ul is required for the test.
Kit Components: The kit contains a pre-coated 96-well plate, a standard substance, an anti-mouse CXCL2/MIP-2 detection antibody, a dilution buffer, color developing solution (A, B), a washing solution, a stop solution, streptavidin-horseradish peroxidase (SA-HRP), a sealing film, and an instruction manual.
Sensitivity: The sensitivity of the kit can be found in the provided instructions.
Detection Range: The kit provides a detection range from 15.6-1000 pg/mL.
Recovery Range: The recovery range of the kit is between 96-103%.
Storage Method: The kit should be stored at 2-8°C.
Background: CXCL2, also known as macrophage inflammatory protein 2-alpha (MIP2-alpha), growth regulatory protein beta (Gro-beta), and Gro-oncogene-2 (Gro-2), belongs to the CXC chemokine family. It shares 90% homology with CXCL1. CXCL2 is secreted by monocytes and macrophages, and it exhibits a chemotactic effect on polymorphonuclear leukocytes and hematopoietic stem cells. The CXCL2 gene is located on human chromosome 4 within the CXC chemokine cluster. It interacts with cell surface chemokine receptors known as CXCR2 to mobilize cells. The human CXCL2/GRO beta consists of 107 amino acids (aa), with an estimated molecular weight of 11 kDa. The mouse and rat orthologs show 70% and 71% amino acid sequence identity to the human protein, respectively. Proteolytic cleavage of the N-terminal amino acids 1-4 of CXCL2/GRO beta can enhance its hematopoietic biological activity. CXCL2/GRO beta is produced by various cell types, including monocytes and macrophages at sites of inflammation, and has a chemotactic effect on granulocytes, including neutrophils.

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