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Rabbit Anti-wnt1 Polyclonal Antibody#abs131412

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Description

Catalog-specification	Delivery time	USD price
abs131412-50ug	1-2 Weeks	201.0
abs131412-100ug	1-2 Weeks	301.0

Overview
catalog	abs131412
Description	This molecule acts as a ligand for the frizzled family of seven transmembrane receptors and has been implicated in numerous developmental processes. It has also been identified as a ligand for the coreceptor RYK, which can trigger the Wnt signaling pathway. This molecule is likely to be important in the development of the central nervous system and may signal over a limited range, encompassing only a few cell diameters. Overall, this developmental protein appears to be a crucial signaling molecule with diverse functions in various biological contexts.
Other names	Int1, also known as Wnt1, is a proto-oncogene that plays a crucial role in various cellular processes, including cell growth and differentiation. The protein product of Int1, Proto-oncogene protein Wnt 1, is a member of the wingless-type MMTV integration site family and is a homolog of Proto-oncogene Int-1. This oncogene is highly conserved across different species and is involved in the development of the nervous system, mammary gland, and other tissues. Dysregulation of Int1 can lead to the formation of various types of cancers, including breast cancer. Understanding the mechanisms of Int1 signaling is vital for the development of novel cancer therapies.
Source	Rabbit
Specificity	The total endogenous levels of wnt1 can be detected using the wnt1 antibody. To elaborate, the antibody specifically targets and identifies the presence of wnt1 protein in the biological samples. This indication of total wnt1 allows for further research and analysis of its functions and role in various biological processes.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Pig;Rabbit;Sheep;Dog;Bovine;Horse;
Antigen	wnt1
Application	The dilution ratios for WB are 1:500 to 1:2000, for IHC they are 1:50 to 1:200, for IF they are 1:100 to 1:500, and for ELISA (peptide) they are 1:20000 to 1:40000. Please rearrange the provided information to generate a highly similar content.
Immunogen	A synthesized peptide derived from human wnt1.
MW	41 kDa
Properties
Concentration	1mg/ml
purification	To purify the antiserum, SulfoLink™ Coupling Resin was utilized for peptide affinity chromatography. This process was employed to ensure that the antiserum was highly purified and optimized for its intended use. The use of this resin resulted in an efficient purification method that allowed for maximum yield of high-quality antiserum for further analysis and experimentation. Overall, the peptide affinity chromatography using SulfoLink™ Coupling Resin proved to be a valuable technique in preparing the antiserum for various applications.
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Ligand for members of the frizzled family of seven transmembrane receptors. In some developmental processes, is also a ligand for the coreceptor RYK, thus triggering Wnt signaling. Probable developmental protein. May be a signaling molecule important in CNS development. Is likely to signal over only few cell diameters. Has a role in osteoblast function and bone development.
Posttranslational modification	Palmitoleylation is required for efficient binding to frizzled receptors. Palmitoleylation is necessary for proper trafficking to cell surface (Probable). Depalmitoleylated by NOTUM, leading to inhibit Wnt signaling pathway (By similarity).
Celluar localization	Endoplasmic reticulum;Extracellular region or secreted;Golgi apparatus;Plasma Membrane;
UniPort	P04628