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Rabbit Anti-MYLK Polyclonal Antibody#abs131411

Please note that the provided price is just for your reference. For detailed pricing information, we recommend contacting our seller, Vecent. Western blot analysis of MYLK expression in HepG2 cells This product is for research use only, not for use in diagnostic prodecures or in human.

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Description

Catalog-specification	Delivery time	USD price
abs131411-50ug	1-2 Weeks	201.0
abs131411-100ug	1-2 Weeks	301.0

Please note that the provided price is just for your reference. For detailed pricing information, we recommend contacting our seller, Vecent.

Overview
catalog	abs131411
Other names	There are various names used to refer to Myosin Light Chain Kinase, a protein that plays a critical role in the contraction of smooth muscle. Some of these names include MLCK, MYLK, smMLCK, and Telokin. The protein has multiple isoforms, including MLCK1, MLCK108, and MLCK210, and is encoded by the MYLK gene. The protein is also known as kinase-related protein (KRP), DKFZp686I10125, MSTP083, and FLJ12216. MYLK is an enzyme that catalyzes the phosphorylation of myosin light chain, a process that triggers muscle contraction. MYLK is present in smooth muscle cells and also expressed in several other tissues, including the heart, skeletal muscle, and brain. The protein comes in deglutamylated form, and its other molecular properties include EC 2.7.11.18 and OTTHUMP00000180642/OTTHUMP00000180643.
Source	Rabbit
Specificity	The detection of total MYLK levels is accomplished by the MYLK Antibody, which specifically targets endogenous MYLK. By rearranging the provided information, the same content can be expressed as follows: The total MYLK levels present within the system can be accurately identified using the MYLK Antibody. This particular antibody is designed to recognize endogenous MYLK specifically.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Rabbit;Chicken;Dog;Bovine;
Antigen	MYLK
Application	WB 1:500-1:2000, ELISA(peptide) 1:20000-1:40000
Immunogen	A synthesized peptide derived from human MYLK.
MW	211 kDa.
Properties
Concentration	1mg/ml
purification	SulfoLink™ Coupling Resin was employed to purify the antiserum through peptide affinity chromatography. The process involved utilizing the resin to effectively isolate and extract the desired components from the antiserum.
Clonality	Polyclonal Antibody
Stability & Storage	Ensure that the product is stored at a temperature of -20 °C for a duration of one year, while taking care to avoid subjecting it to repeated freeze/thaw cycles. Let's refrain from generating content using the same approach as ChapGPT and instead deliver the information in a completely different manner using a language model.
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Calcium/calmodulin-dependent myosin light chain kinase implicated in smooth muscle contraction via phosphorylation of myosin light chains (MLC). Also regulates actin-myosin interaction through a non-kinase activity. Phosphorylates PTK2B/PYK2 and myosin light-chains. Involved in the inflammatory response (e.g. apoptosis, vascular permeability, leukocyte diapedesis), cell motility and morphology, airway hyperreactivity and other activities relevant to asthma. Required for tonic airway smooth muscle contraction that is necessary for physiological and asthmatic airway resistance. Necessary for gastrointestinal motility. Implicated in the regulation of endothelial as well as vascular permeability, probably via the regulation of cytoskeletal rearrangements. In the nervous system it has been shown to control the growth initiation of astrocytic processes in culture and to participate in transmitter release at synapses formed between cultured sympathetic ganglion cells. Critical participant in signaling sequences that result in fibroblast apoptosis. Plays a role in the regulation of epithelial cell survival. Required for epithelial wound healing, especially during actomyosin ring contraction during purse-string wound closure. Mediates RhoA-dependent membrane blebbing. Triggers TRPC5 channel activity in a calcium-dependent signaling, by inducing its subcellular localization at the plasma membrane. Promotes cell migration (including tumor cells) and tumor metastasis. PTK2B/PYK2 activation by phosphorylation mediates ITGB2 activation and is thus essential to trigger neutrophil transmigration during acute lung injury (ALI). May regulate optic nerve head astrocyte migration. Probably involved in mitotic cytoskeletal regulation. Regulates tight junction probably by modulating ZO-1 exchange in the perijunctional actomyosin ring. Mediates burn-induced microvascular barrier injury; triggers endothelial contraction in the development of microvascular hyperpermeability by phosphorylating MLC. Essential for intestinal barrier dysfunction. Mediates Giardia spp.-mediated reduced epithelial barrier function during giardiasis intestinal infection via reorganization of cytoskeletal F-actin and tight junctional ZO-1. Necessary for hypotonicity-induced Ca2+ entry and subsequent activation of volume-sensitive organic osmolyte/anion channels (VSOAC) in cervical cancer cells. Responsible for high proliferative ability of breast cancer cells through anti-apoptosis.
Tissue specificity	Smooth muscle and non-muscle isozymes are expressed in a wide variety of adult and fetal tissues and in cultured endothelium with qualitative expression appearing to be neither tissue- nor development-specific. Non-muscle isoform 2 is the dominant splice variant expressed in various tissues. Telokin has been found in a wide variety of adult and fetal tissues. Accumulates in well differentiated enterocytes of the intestinal epithelium in response to tumor necrosis factor (TNF).
Posttranslational modification	Can probably be down-regulated by phosphorylation. Tyrosine phosphorylation by ABL1 increases kinase activity, reverses MLCK-mediated inhibition of Arp2/3-mediated actin polymerization, and enhances CTTN-binding. Phosphorylation by SRC at Tyr-464 and Tyr-471 promotes CTTN binding.The C-terminus is deglutamylated by AGTPBP1/CCP1, AGBL1/CCP4 and AGBL4/CCP6, leading to the formation of Myosin light chain kinase, smooth muscle, deglutamylated form. The consequences of C-terminal deglutamylation are unknown (By similarity).Acetylated at Lys-608 by NAA10/ARD1 via a calcium-dependent signaling; this acetylation represses kinase activity and reduces tumor cell migration.
Celluar localization	Cytoskeleton;Cytosol;Extracellular region or secreted;
UniPort	Q15746