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Rabbit Anti-CRHR1 Polyclonal Antibody#abs131407

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Description

Catalog-specification	Delivery time	USD price
abs131407-100ug	1-2 Weeks	301.0
abs131407-50ug	1-2 Weeks	201.0

Overview
catalog	abs131407
Description	This receptor exhibits a strong affinity for corticotropin releasing factor and serves as a binding site for this hormone. It effectively binds to CRF with high-affinity. The functioning of this receptor relies on G proteins that facilitate the activation of adenylyl cyclase, leading to subsequent cellular responses.
Other names	Corticotropin releasing factor type 1 receptor, also known as corticotropin releasing hormone receptor 1, is denoted by various abbreviations such as CRF R, crf receptor 1, crf receptor type 1, crf type 1, CRF-R, CRF-R-1, CRF-R1, CRF1, CRFR, CRFR-1, CRFR1, CRFR1_HUMAN, CRH-R-1, CRH-R1, CRH-R1h, CRHR, Crhr1, CRHR1f, CRHR1L.
Source	Rabbit
Specificity	The CRHR1 Antibody has the ability to identify total CRHR1 levels that exist naturally within the body. By reorganizing the original content, a highly similar message can be conveyed while still maintaining the same underlying information.
Species Reactivity	Human;Mouse;Rat
Antigen	CRHR1
Application	For western blot analysis, a dilution range of 1:500 to 1:2000 is recommended. When performing immunofluorescence staining, it is advised to use a dilution range of 1:100 to 1:500. For immunohistochemistry studies, a dilution of 1:200 is recommended. For enzyme-linked immunosorbent assay (ELISA) experiments using peptide antigens, it is recommended to use a dilution range of 1:20000 to 1:40000. It is important to note that the specific antibody and target antigen may require optimization of dilution factors to achieve optimal results.
Immunogen	A synthesized peptide derived from human CRHR1.
MW	51 kDa
Properties
Concentration	1mg/ml
purification	Using SulfoLink™ Coupling Resin, the antiserum underwent peptide affinity chromatography purification. Similarly, the purification process involved separating and isolating the desired antibodies based on their specific binding properties to the resin-bound peptides.
Clonality	Polyclonal Antibody
Stability & Storage	To maintain the quality, store for one year at -20 °C. Prevent repeated freeze/thaw cycles from occurring. Repeatedly freezing and thawing should be avoided.
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	G-protein coupled receptor for CRH (corticotropin-releasing factor) and UCN (urocortin). Has high affinity for CRH and UCN. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and down-stream effectors, such as adenylate cyclase. Promotes the activation of adenylate cyclase, leading to increased intracellular cAMP levels. Inhibits the activity of the calcium channel CACNA1H. Required for normal embryonic development of the adrenal gland and for normal hormonal responses to stress. Plays a role in the response to anxiogenic stimuli.
Tissue specificity	Predominantly expressed in the cerebellum, pituitary, cerebral cortex and olfactory lobe.
Posttranslational modification	C-terminal Ser or Thr residues may be phosphorylated.Phosphorylation at Ser-330 by PKA prevents maximal coupling to Gq-protein, and thereby negatively regulates downstream signaling.
Celluar localization	Endosome;Plasma Membrane;
UniPort	P34998