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Rabbit Anti-AGER(RAGE) Polyclonal Antibody#abs131405

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Description

Catalog-specification	Delivery time	USD price
abs131405-100ug	1-2 Weeks	301.0
abs131405-50ug	1-2 Weeks	201.0

Overview
catalog	abs131405
Description	Age is responsible for mediating interactions of advanced glycosylation end products. These end products, which are proteins that have been glycosylated nonenzymatically, tend to accumulate in vascular tissue as individuals age. However, the accumulation rate is even higher in individuals with diabetes. Age plays a crucial role as a mediator in both acute and chronic vascular inflammation, particularly in conditions like atherosclerosis and as a complication of diabetes.
Other names	The Ager gene, also known as MGC2235 or RAGE_HUMAN, codes for the advanced glycosylation end product-specific receptor. This receptor is integral in recognizing and binding to advanced glycosylation end products. Hence, it is also referred to as the receptor for advanced glycosylation end products.
Source	Rabbit
Specificity	The AGER (RAGE) Antibody can identify the total amount of AGER (RAGE) present in the body. This antibody is capable of detecting the endogenous levels of AGER (RAGE) in the body. To ensure that the generated content is based on the information provided in the original text, a highly similar content can be produced by rephrasing the original sentence.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Rabbit
Antigen	AGER
Application	IHC 1:50-1:200, WB 1:500-1:2000, IF 1:100-1:500, and ELISA (peptide) 1:20000-1:40000 are the recommended dilutions for various laboratory techniques. These dilutions are crucial for achieving optimal results in experiments. For Western blotting (WB), a dilution range of 1:500-1:2000 is commonly used, while immunohistochemistry (IHC) requires a dilution of 1:50-1:200. In the case of immunofluorescence (IF), the suggested dilution is 1:100-1:500, and for ELISA using peptides, a dilution range of 1:20000-1:40000 is preferred. It is essential to follow these dilutions carefully to ensure accurate and reliable data during laboratory investigations.
Immunogen	One can create a peptide that has been synthesized and is based on human AGER (RAGE). The synthesized version is designed to closely resemble the original protein in structure and function. This type of synthesized peptide is an important tool in studying the biological roles played by the AGER (RAGE) protein.
MW	42 kDa
Properties
Concentration	1mg/ml
purification	Using SulfoLink™ Coupling Resin, peptide affinity chromatography was employed to purify the antiserum.
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Mediates interactions of advanced glycosylation end products (AGE). These are nonenzymatically glycosylated proteins which accumulate in vascular tissue in aging and at an accelerated rate in diabetes. Acts as a mediator of both acute and chronic vascular inflammation in conditions such as atherosclerosis and in particular as a complication of diabetes. AGE/RAGE signaling plays an important role in regulating the production/expression of TNF-alpha, oxidative stress, and endothelial dysfunction in type 2 diabetes. Interaction with S100A12 on endothelium, mononuclear phagocytes, and lymphocytes triggers cellular activation, with generation of key proinflammatory mediators. Interaction with S100B after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling (By similarity). Receptor for amyloid beta peptide. Contributes to the translocation of amyloid-beta peptide (ABPP) across the cell membrane from the extracellular to the intracellular space in cortical neurons. ABPP-initiated RAGE signaling, especially stimulation of p38 mitogen-activated protein kinase (MAPK), has the capacity to drive a transport system delivering ABPP as a complex with RAGE to the intraneuronal space. Can also bind oligonucleotides.
Tissue specificity	Endothelial cells.
Celluar localization	Extracellular region or secreted;Nucleus;Plasma Membrane;
UniPort	Q15109