Rabbit Anti-TRI18 Polyclonal Antibody #abs130257

abs130257-50ug

1-2 Weeks

201

abs130257-100ug

1-2 Weeks

301

Description

MID1 is a member of the TRIM/RBCC family and may exhibit E3 ubiquitin ligase activity that targets the catalytic subunit of protein phosphatase 2 for degradation. An alternative tissue-specific promoter, which is derived from a retroviral element, regulates the expression of this gene. The LTR of an HERV-E element further amplifies the expression in embryonic kidney and placenta. Moreover, MID1 forms homodimers or heterodimers with MID2 and interacts with IGBP1. Two isoforms of this protein are produced via alternative splicing mechanisms.

Other names

Midline-1 (also known as Mid1 or OGS1) is a protein involved in the Opitz/BBB syndrome. It functions as an E3 ubiquitin-protein ligase and is characterized by its RING finger domain. In mice, it has a homolog called Finger on X and Y (FXY). Midline-1 is also referred to as GBBB1 or RNF59, and it is encoded by the TRIM18 gene.
This protein is believed to have a role in transcription regulation and is considered a putative transcription factor (XPRF). It contains a tripartite motif, making it a member of the tripartite motif-containing protein family. The zinc finger domain on X and Y (ZNFXY) is a conserved feature of this protein.
To summarize, Midline-1 is a ubiquitin ligase protein that plays a crucial role in Opitz/BBB syndrome. It has various names and aliases such as GBBB1, RNF59, Mid1, and OGS1. Additionally, it is associated with the tripartite motif-containing protein family and contains a zinc finger domain encoded by the TRIM18 gene.

Source

Rabbit

Specificity

The endogenous levels of total TRI18 can be accurately detected using the TRI18 antibody. It is capable of identifying the presence of TRI18 in a sample, ensuring reliable measurements. By utilizing the TRI18 antibody, researchers can gain insights into the quantitative analysis of this protein. The antibody's specificity and sensitivity make it a valuable tool in various scientific investigations related to TRI18.

Species Reactivity

Human;Mouse;Rat

Application

。（IHC），1:501:200。/（IF/ICC），1:1001:500。（ELISA）（），1:200001:40000。，。

Immunogen

A synthesized peptide derived from human TRI18

Properties

Concentration

1mg/ml

Purification

Affinity-chromatography

Clonality

Polyclonal Antibody

Stability & Storage

One year storage is recommended at -20 °C. Make sure to prevent repeated freezing and thawing cycles. It is important to note that the generated content should not be a conversation but a speech based on the language model generating text in a completely different manner from ChapGPT.

Storage buffer

The Rabbit IgG is stored in phosphate buffered saline with a pH of 7.4, 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It should be kept at a temperature of -20 °C. This product remains stable for a period of 12 months from the date of receipt. Please note that the requested content has been rearranged from the original text while ensuring that the information provided remains the same.

Target

Background

Has E3 ubiquitin ligase activity towards IGBP1, promoting its monoubiquitination, which results in deprotection of the catalytic subunit of protein phosphatase PP2A, and its subsequent degradation by polyubiquitination.

Tissue specificity

In the fetus, highest expression found in kidney, followed by brain and lung. Expressed at low levels in fetal liver. In the adult, most abundant in heart, placenta and brain.

Posttranslational modification

Phosphorylated on serine and threonine residues.

Celluar localization

Cytoskeleton;Cytosol;

UniPort

O15344