Rabbit Anti-Cyclin L1 Polyclonal Antibody #abs130172

abs130172-50ug

1-2 Weeks

201

abs130172-100ug

1-2 Weeks

301

Description

Cyclin L1, also known as CCNL1, is a crucial member of the cyclin protein family. It plays a significant role in pre-mRNA processing by associating with the PITSLRE kinase. This partnership ensures efficient and accurate processing of precursor mRNA molecules. In summary, CCNL1 is a central player in the intricate machinery responsible for regulating gene expression at the pre-mRNA stage.

Other names

Cyclin L, also known as Cyclin L1 or Cyclin-L, is a protein encoded by the CCNL1 gene in humans. It belongs to the Cyclin-Box family of proteins and is essential for proper cell division and growth. Cyclin L exhibits gamma subunit activity and plays a crucial role in the regulation of RNA polymerase II transcription. This protein is primarily expressed in testis and multiple types of cancer cells. In conclusion, Cyclin L is a critical protein that could be a potential target for cancer therapy.

Source

Rabbit

Specificity

The detection of total Cyclin L1 is possible using the Cyclin L1 antibody, which is capable of recognizing endogenous levels of the protein. A closely similar content can be produced by rephrasing the original information while retaining its meaning.

Species Reactivity

Human;Mouse;Rat

Application

For Western blotting, the recommended dilution range for this antibody is between 1:500 and 1:3000. For immunohistochemistry, researchers should use a dilution range between 1:50 and 1:200. When performing ELISA testing with a peptide, a dilution range of 1:20000-1:40000 is appropriate. It is important to follow these recommended dilution ranges to ensure accurate and reliable results.

Immunogen

A synthesized peptide derived from human Cyclin L1

Properties

Concentration

1mg/ml

Purification

Affinity-chromatography

Clonality

Polyclonal Antibody

Stability & Storage

To ensure optimal storage conditions, it is recommended that the product be kept at a temperature of -20 °C for a duration of one year. It is important to avoid repeated cycles of freezing and thawing as this can degrade the product's quality. It is advisable to follow these guidelines to maintain the integrity and efficacy of the product.

Storage buffer

The rabbit IgG solution is prepared in a phosphate-buffered saline (PBS) with a pH of 7.4. It contains 150mM sodium chloride (NaCl), 0.02% sodium azide, and 50% glycerol. This solution should be stored at -20°C. It has a shelf life of 12 months from the date of receipt. Please note that the generated content may vary significantly from the original text.

Target

Background

This protein is involved in pre-mRNA splicing and is known to function in conjunction with cyclin-dependent kinases (CDKs) (PubMed:18216018). Its activity is inhibited by the CDK-specific inhibitor CDKN1A/p21 (PubMed:11980906). Additionally, it is believed to have a role in regulating RNA polymerase II (pol II). Interestingly, studies have suggested that it could potentially be a candidate proto-oncogene in head and neck squamous cell carcinomas (HNSCC) (PubMed:12414649, PubMed:15700036).
In summary, this protein plays a crucial role in pre-mRNA splicing and interacts with CDKs. Its function is impeded by CDKN1A/p21 and it may be involved in regulating RNA polymerase II. Notably, it has been proposed as a potential proto-oncogene in HNSCC.

Tissue specificity

Head and neck squamous cell carcinomas (HNSCC) commonly exhibit widespread overexpression in primary tumors. The overexpression in these tumors is widely acknowledged and discussed.

Celluar localization

Nucleus;

UniPort

Q9UK58