Phospho-Fyn (Tyr530) Rabbit Polyclonal Antibody#abs130730

abs130730-50ug

1-2 Weeks

201

abs130730-100ug

1-2 Weeks

301

Description

Maintenance of chromosomal stability requires the presence of FANCD2. It plays a crucial role in ensuring accurate and efficient pairing of homologous chromosomes during meiosis. Additionally, FANCD2 is involved in the repair of DNA double-strand breaks through both homologous recombination and single-strand annealing mechanisms.
In response to DNA damage, FANCD2 is responsible for activating S phase and G2 phase checkpoints, which are essential for proper cell cycle progression. It also has a vital function in preventing the breakage and loss of missegregating chromatin after cell division, particularly when cells experience replication stress.
Furthermore, FANCD2 is required for targeting or stabilizing the protein BLM at abnormal structures induced by replicative stress. This interaction is crucial for maintaining genomic integrity and ensuring proper DNA repair processes.

Other names

The FYN proto-oncogene is an important gene involved in cell signaling pathways. It is also known as the Fyn protein tyrosine kinase or p59-Fyn. FYN is related to other well-known oncogenes such as SRC, FGR, and YES. In humans, this gene is encoded by the FYN_HUMAN gene. It serves as a regulator of calcium influx induced by OKT3. Additionally, FYN plays a role in various cellular processes, including protein phosphorylation.
The protein encoded by FYN is a tyrosine kinase, belonging to the Src family of kinases. It is involved in intracellular signaling cascades that regulate cell growth, differentiation, and survival. FYN has been associated with several diseases and disorders, including cancers and neurological conditions.
One of the notable features of FYN is its similarity to the c-Fyn proto-oncogene. This proto-oncogene, also known as Proto-oncogene Syn, has been implicated in promoting cancer development. The FYN gene shares important functional and structural similarities with c-Fyn and is considered a proto-oncogene itself.
Another name for FYN is Syn, which stands for Src yes related novel gene. This alternative name highlights its connection to the Src family of kinases and its novel characteristics. FYN is also referred to as a Src-like kinase due to its resemblance to other members of this kinase family.
Overall, FYN is an essential protein involved in cellular signaling pathways and plays a crucial role in various physiological and pathological processes. Its functions as a tyrosine kinase make it an attractive target for therapeutic interventions in various diseases.

Source

Rabbit

Specificity

The Phospho-Fyn (Tyr530) Antibody can specifically identify Fyn protein that has been phosphorylated at Tyrosine 530. This antibody is able to recognize endogenous levels of Fyn only when it is in a phosphorylated state at this specific site. By using this antibody, researchers can study the functionality and localization of phosphorylated Fyn protein, which plays important roles in various signaling pathways. The specificity of this antibody makes it a valuable tool in investigating the regulation of Fyn activity in cellular processes.

Predictive reaction species

Pig;Rabbit;Sheep;Dog;Bovine

Application

The dilution range for this antibody is between 1:500 to 1:2000 for Western blotting, 1:50 to 1:200 for Immunohistochemistry, 1:100 to 1:500 for Immunofluorescence/Immunocytochemistry, and 1:20000-1:40000 for ELISA testing with peptide. It is important to maintain proper dilution ratios to ensure the best possible results.

A peptide synthesized from the human Fyn protein at the Tyrosine 530 phosphorylation site is now available. This molecule is highly similar to the original Fyn sequence and can be used in various research applications. Its unique structure may provide insights into the regulation of Fyn activity and its role in cellular signaling pathways. With this peptide, researchers can now study the potential therapeutic benefits of targeting Fyn in diseases such as cancer and Alzheimer's.

Properties

1mg/ml

Purification

The purification process of the antibody involved sequential chromatography on both phospho- and non-phospho-peptide affinity columns, resulting in highly purified rabbit serum. The final product, an antibody, was extracted and deemed to be highly similar to the original serum.

Clonality

Polyclonal Antibody

Ensure that the storage temperature is maintained at -20 °C for a period of one year, without any occurrence of repeated freeze/thaw cycles.

Storage buffer

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Target

Background

 Non-receptor tyrosine-protein kinase that plays a role in many biological processes including regulation of cell growth and survival, cell adhesion, integrin-mediated signaling, cytoskeletal remodeling, cell motility, immune response and axon guidance. Inactive FYN is phosphorylated on its C-terminal tail within the catalytic domain. Following activation by PKA, the protein subsequently associates with PTK2/FAK1, allowing PTK2/FAK1 phosphorylation, activation and targeting to focal adhesions. Involved in the regulation of cell adhesion and motility through phosphorylation of CTNNB1 (beta-catenin) and CTNND1 (delta-catenin). Regulates cytoskeletal remodeling by phosphorylating several proteins including the actin regulator WAS and the microtubule-associated proteins MAP2 and MAPT. Promotes cell survival by phosphorylating AGAP2/PIKE-A and preventing its apoptotic cleavage. Participates in signal transduction pathways that regulate the integrity of the glomerular slit diaphragm (an essential part of the glomerular filter of the kidney) by phosphorylating several slit diaphragm components including NPHS1, KIRREL and TRPC6. Plays a role in neural processes by phosphorylating DPYSL2, a multifunctional adapter protein within the central nervous system, ARHGAP32, a regulator for Rho family GTPases implicated in various neural functions, and SNCA, a small pre-synaptic protein. Participates in the downstream signaling pathways that lead to T-cell differentiation and proliferation following T-cell receptor (TCR) stimulation. Also participates in negative feedback regulation of TCR signaling through phosphorylation of PAG1, thereby promoting interaction between PAG1 and CSK and recruitment of CSK to lipid rafts. CSK maintains LCK and FYN in an inactive form. Promotes CD28-induced phosphorylation of VAV1.

Tissue specificity

Isoform 1 is highly expressed in the brain. Isoform 2 is expressed in cells of hemopoietic lineages, especially T-lymphocytes.

Celluar localization

Cytoskeleton;Cytosol;Endosome;Mitochondrion;Nucleus;Plasma Membrane;

P06241