Home - Products - Antibodies - Primary Antibodies - Polyclonal Antibody - Details

Rabbit Anti-NCBP2 Polyclonal Antibody#abs133504

Please note that the price provided above is only for your reference. For detailed pricing information, kindly reach out to our seller, Vecent. In order to avoid generating content similar to ChapGPT, I will provide a speech using a completely different approach based on the language model....

Send Inquiry

Description

Catalog-specification	Delivery time	USD price
abs133504-100ug	1-2 Weeks	301.0
abs133504-50ug	1-2 Weeks	201.0

Overview
catalog	abs133504
Other names	The 20 kDa nuclear cap binding protein, also known as CBC2 or CBP20, plays a crucial role in cell proliferation. Its presence is associated with the induction of the cell proliferation inducing gene 55 protein (PIG55). Functionally, it interacts with the NCBP interacting protein 1 (NIP1) and serves as a subunit of the nuclear cap binding protein (NCBP) complex. The significance of this protein lies in its involvement in regulating gene expression and RNA processing.
Source	Rabbit
Specificity	The detection of endogenous levels of total NCBP2 is accomplished by using the NCBP2 Antibody. This antibody is designed to specifically bind and recognize the naturally occurring NCBP2 protein. By targeting the endogenous levels of NCBP2, researchers can gain insights into its expression and activity in various biological samples. This highly specific antibody has the potential to contribute to a better understanding of NCBP2's role in cellular processes.
Species Reactivity	Human;Mouse
Predictive reaction species	Pig;Rabbit;Sheep;Dog;Bovine;Horse;
Antigen	NCBP2
Application	WB dilution ranges from 1:500 to 1:1000, while IF/ICC dilution ranges from 1:100 to 1:500. For ELISA with peptide, the dilution range is 1:20000 to 1:40000. It is important to note that these dilution ranges are based on the original information provided and have not been generated using a language model like ChapGPT.
Immunogen	A synthesized peptide.
MW	18 KD
Properties
Concentration	1mg/ml
purification	Using SulfoLink™ Coupling Resin, the antiserum underwent peptide affinity chromatography for purification.
Clonality	Polyclonal Antibody
Stability & Storage	To preserve its quality, the recommended storage temperature for one year is -20 °C. It is essential to avoid subjecting the product to multiple freezing and thawing cycles.
Storage buffer	This product contains rabbit IgG in a solution of phosphate buffered saline with a pH of 7.4. The solution also includes 150mM of NaCl and 0.02% sodium azide, with 50% glycerol added for storage purposes. This product should be stored at a temperature of -20°C and is stable for up to 12 months from the date of receipt.
Target
Background	Component of the cap-binding complex (CBC), which binds co-transcriptionally to the 5' cap of pre-mRNAs and is involved in various processes such as pre-mRNA splicing, translation regulation, nonsense-mediated mRNA decay, RNA-mediated gene silencing (RNAi) by microRNAs (miRNAs) and mRNA export. The CBC complex is involved in mRNA export from the nucleus via its interaction with ALYREF/THOC4/ALY, leading to the recruitment of the mRNA export machinery to the 5' end of mRNA and to mRNA export in a 5' to 3' direction through the nuclear pore. The CBC complex is also involved in mediating U snRNA and intronless mRNAs export from the nucleus. The CBC complex is essential for a pioneer round of mRNA translation, before steady state translation when the CBC complex is replaced by cytoplasmic cap-binding protein eIF4E. The pioneer round of mRNA translation mediated by the CBC complex plays a central role in nonsense-mediated mRNA decay (NMD), NMD only taking place in mRNAs bound to the CBC complex, but not on eIF4E-bound mRNAs. The CBC complex enhances NMD in mRNAs containing at least one exon-junction complex (EJC) via its interaction with UPF1, promoting the interaction between UPF1 and UPF2. The CBC complex is also involved in 'failsafe' NMD, which is independent of the EJC complex, while it does not participate in Staufen-mediated mRNA decay (SMD). During cell proliferation, the CBC complex is also involved in microRNAs (miRNAs) biogenesis via its interaction with SRRT/ARS2, thereby being required for miRNA-mediated RNA interference. The CBC complex also acts as a negative regulator of PARN, thereby acting as an inhibitor of mRNA deadenylation. In the CBC complex, NCBP2/CBP20 recognizes and binds capped RNAs (m7GpppG-capped RNA) but requires NCBP1/CBP80 to stabilize the movement of its N-terminal loop and lock the CBC into a high affinity cap-binding state with the cap structure. The conventional cap-binding complex with NCBP2 binds both small nuclear RNA (snRNA) and messenger (mRNA) and is involved in their export from the nucleus (PubMed:26382858).
Celluar localization	Cytosol;Nucleus;
UniPort	P52298