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Rabbit Anti-VCL Polyclonal Antibody#abs136116

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Description

Catalog-specification	Delivery time	USD price
abs136116-100ug	1-2 Weeks	301.0
abs136116-50ug	1-2 Weeks	201.0

Overview
catalog	abs136116
Description	Vinculin, an essential cytoskeletal protein, is involved in regulating focal adhesions and embryonic development (1-4). It consists of three main structural domains: an amino-terminal head, a proline-rich region, and a carboxy-terminal tail (1). In its inactive state, the head and tail domains of vinculin interact to form a closed confirmation. However, when vinculin is phosphorylated at specific sites such as Ser1033, Ser1045, Tyr100, and Tyr1065 by PKC-α and Src kinases, and when phospholipids bind to the tail domain, this interaction weakens (5,6). This alteration allows vinculin to adopt an open and active form, which then localizes to focal adhesions. In this active state, vinculin is believed to play a crucial role in anchoring F-actin to the cell membrane and regulating cell migration (2). Furthermore, the disruption of the head-tail interaction due to binding with other proteins like talin, α-actinin, and paxillin initiates a conformational change from the inactive to active state (2,4). Notably, deficiencies in vinculin expression result in reduced cell adhesion and increased cell motility, suggesting its potential involvement in metastatic growth (7,8). Recent studies have even shown a correlation between decreased vinculin expression and elevated carcinogenesis and metastasis in colorectal carcinoma (9). In summary, vinculin is a critical cytoskeletal protein essential for focal adhesion regulation and embryonic development. Its structural domains and phosphorylation play a crucial role in transitioning between inactive and active states, impacting F-actin anchoring, cell migration, and potentially metastatic growth in certain cancers.
Other names	Hel114, also known as Vinculin, is a protein found in the luminal region of the epididymis. It is encoded by the OTTHUMP00000019861 and OTTHUMP00000019862 genes. Vinculin, also referred to as MVCL, plays a crucial role in cell adhesion and cytoskeletal organization. This protein is involved in various cellular processes and is essential for maintaining the structural integrity of cells. It is an important component of focal adhesions and interacts with other proteins to regulate cell motility. Vinculin, with its various aliases including CMD1W and CMH15, is a key protein in the human body and is known for its role in facilitating cell communication and tissue development.
Source	Rabbit
Specificity	Endogenous levels of total VCL can be effectively detected by the VCL Antibody. The content should be rearranged to maintain the same meaning while utilizing a different structure.
Species Reactivity	Human;Mouse;Rat
Antigen	VCL
Application	For Western blotting applications, dilute the primary antibody at a ratio of 1:500 to 1:2000. For immunohistochemistry, a dilution ratio of 1:50 to 1:200 is recommended. When using an ELISA assay with a peptide substrate, the antibody should be diluted at a range of 1:20000 to 1:40000. It is important to note that these dilution ranges are based on the original text information and should be followed accordingly for optimal results.
Immunogen	A synthesized peptide derived from human VCL.
MW	124kDa
Properties
Concentration	1mg/ml
purification	The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion.
Tissue specificity	Metavinculin is muscle-specific.
Posttranslational modification	Phosphorylated; on serines, threonines and tyrosines. Phosphorylation on Tyr-1133 in activated platelets affects head-tail interactions and cell spreading but has no effect on actin binding nor on localization to focal adhesion plaques (By similarity).Acetylated; mainly by myristic acid but also by a small amount of palmitic acid.
Celluar localization	Cytoskeleton;Cytosol;Extracellular region or secreted;Plasma Membrane;
UniPort	P18206