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Rabbit Anti-POLR2E Polyclonal Antibody#abs136115

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Description

Catalog-specification	Delivery time	USD price
abs136115-100ug	1-2 Weeks	301.0
abs136115-50ug	1-2 Weeks	201.0

Overview
catalog	abs136115
Description	The RNA polymerase II is the enzyme responsible for transcribing eukaryotic messenger RNA, and it consists of several subunits, with the fifth largest subunit being encoded by this gene. Interestingly, this subunit is present in higher amounts compared to the other subunits, and it is shared by other DNA-directed RNA polymerases. Recent studies have shown that this subunit can bind to a hepatitis virus transactivating protein, suggesting that it plays a crucial role in regulating transcriptional activators in the cell. Moreover, it is worth noting that a pseudogene of this gene is located on chromosome 11, further highlighting its importance in regulating gene expression in the cell. With its crucial role in synthesizing messenger RNA, this gene has been the subject of extensive research, and it is hoped that further studies will shed more light on its specific functions and how it contributes to normal cellular processes.
Other names	The following is a rearranged version of the content provided: - DNA-directed RNA polymerase II subunit E (Polr2e) is an essential component of the RNA polymerase II complex, which is responsible for transcribing DNA into RNA molecules. - It is also known by various names such as DNA-directed RNA polymerase II 23 kDa polypeptide, DNA-directed RNA polymerase II subunit E, and DNA-directed RNA polymerase II polypeptide E. - This subunit plays a crucial role in facilitating the binding of other subunits to the RNA polymerase II complex. - It is encoded by the RPABC1 gene and is found in organisms ranging from humans (hsRPB5) to yeast (RPB5 homolog). - The importance of this subunit is evident as it is required for the proper functioning of RNA polymerases I, II, and III. - Mutations in the RPABC1 gene can lead to various disorders and diseases, highlighting the significance of this subunit in gene expression. - The DNA-directed RNA polymerase II 23 kDa polypeptide is often referred to as hRPB25 or XAP4. - Its molecular weight is approximately 25 kDa, and it plays a crucial role in the transcription process. - The precise mechanisms by which this subunit functions are still being investigated, but it is clear that it is essential for transcriptional activity.
Source	Rabbit
Specificity	The POLR2E antibody is capable of detecting total levels of endogenous POLR2E. This means that the antibody can accurately identify and measure the amount of POLR2E present within the cells or tissue being studied. With its ability to detect total POLR2E, this antibody is a valuable tool for researchers looking to study the function and role of this important protein. By using this antibody, researchers can gain a better understanding of the complex processes in which POLR2E is involved, as well as its potential contribution to disease and pathology.
Species Reactivity	Human;Mouse;Rat
Antigen	POLR2E
Application	For Western blotting, the recommended dilution range is between 1:500 to 1:2000. In order to achieve the best results in immunohistochemistry, a dilution range of 1:50 to 1:200 is recommended. For immunofluorescence and immunocytochemistry, it is recommended to dilute the antibody between 1:100 to 1:500. When using the ELISA technique with a peptide, it is recommended to dilute the antibody between 1:20000 to 1:40000.
Immunogen	A synthesized peptide derived from human POLR2E.
MW	24kDa
Properties
Concentration	1mg/ml
purification	The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and small RNAs, such as 5S rRNA and tRNAs, respectively. Pol II is the central component of the basal RNA polymerase II transcription machinery. Pols are composed of mobile elements that move relative to each other. In Pol II, POLR2E/RPB5 is part of the lower jaw surrounding the central large cleft and thought to grab the incoming DNA template. Seems to be the major component in this process (By similarity).
Celluar localization	Cytosol;Nucleus;
UniPort	P19388