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Rabbit Anti-14-3-3 Epsilon Polyclonal Antibody#abs133793

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Description

Catalog-specification	Delivery time	USD price
abs133793-100ug	1-2 Weeks	301.0
abs133793-50ug	1-2 Weeks	201.0

Overview
catalog	abs133793
Other names	The protein known as 14-3-3E, or tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein epsilon, has various aliases including 14 3 3 E, 14 3 3 epsilon, and YWHAE. It is also referred to as KCIP1, protein kinase C inhibitor protein 1, and MDCR. This protein plays a crucial role in various cellular processes such as signal transduction, cell cycle control, and apoptosis. In addition, 14-3-3E is involved in mitotic regulation and mitochondrial function. It interacts with a diverse range of other proteins and is known to bind phosphoserine or phosphothreonine residues. The protein is made up of two identical monomers, each containing nine anti-parallel alpha-helices that are crucial for mediating protein-protein interactions. Furthermore, 14-3-3E has been implicated in several diseases, including cancer and neurodegenerative disorders, as well as in viral infections. It is expressed in most human tissues, with the highest levels found in the brain, heart, and skeletal muscle. Due to its diverse functions and interactions, 14-3-3E is an intriguing target for future therapeutic interventions.
Source	Rabbit
Specificity	The total 14-3-3 ε present in cells can be accurately detected using the 14-3-3 ε Antibody. This antibody specifically targets the endogenous levels of 14-3-3 ε, allowing for precise detection and analysis. By utilizing this antibody, researchers can gain valuable insights into the cellular processes involving 14-3-3 ε and its potential role in various biological functions.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Pig;Rabbit;Chicken;Sheep;Bovine;Horse;
Antigen	14-3-3 epsilon
Application	For western blotting, the recommended dilution range is 1:500 to 1:1000. If you are using immunofluorescence or immunocytochemistry, a dilution of 1:100 to 1:500 is suggested. If you are conducting an ELISA using a peptide, the recommended dilution range is 1:20000 to 1:40000. It's important to follow these dilution recommendations to achieve optimal results.
Immunogen	A synthesized peptide.
MW	29 KD
Properties
Concentration	1mg/ml
purification	Using SulfoLink™ Coupling Resin, the antiserum underwent peptide affinity chromatography purification. A highly similar version can be generated by rephrasing the original statement: The antiserum was subjected to purification through peptide affinity chromatography, which utilized SulfoLink™ Coupling Resin.
Clonality	Polyclonal Antibody
Stability & Storage	To maintain the quality of the product, it is recommended to store it at a temperature of -20 °C for up to one year. It is important to avoid exposing the product to repeated cycles of freezing and thawing, as this may compromise its integrity. By following these guidelines, you can ensure that the product remains stable and effective for use when needed.
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner (By similarity). Positively regulates phosphorylated protein HSF1 nuclear export to the cytoplasm (PubMed:12917326).
Celluar localization	Cytoskeleton;Cytosol;Extracellular region or secreted;Mitochondrion;Nucleus;
UniPort	P62258