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Rabbit Anti-AOX1 Polyclonal Antibody#abs140835

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Description

Catalog-specification	Delivery time	USD price
abs140835-100ug	1-2 Weeks	301.0
abs140835-50ug	1-2 Weeks	201.0

Overview
catalog	abs140835
Other names	AOH1, also known as aldehyde oxidase 1 or AOX1, is an enzyme known for its role as azaheterocycle hydroxylase. It belongs to the EC 1.2.3.1 enzyme classification. This enzyme plays a crucial role in the oxidation of aldehyde compounds.
Source	Rabbit
Specificity	The AOX1 Antibody is capable of detecting the total AOX1 protein at its natural levels in the body. This means that the antibody can identify and bind to the endogenous amount of AOX1 protein effectively. By utilizing this antibody, researchers can study the overall presence and behavior of AOX1 within biological samples.
Species Reactivity	Human;Mouse;Rat
Antigen	AOX1
Application	The recommended dilution ranges for the antibodies are as follows: 1:500~1:1000 for Western blotting (WB), 1:50~1:200 for immunohistochemistry (IHC), 1:100-1:500 for immunofluorescence/immunocytochemistry (IF/ICC), and 1:20000-1:40000 for enzyme-linked immunosorbent assay (ELISA) using peptide as the antigen. Please note that these dilutions are based on the original text information and should be adjusted according to the specific experimental conditions.
Immunogen	A synthesized peptide.
MW	120 KD
Properties
Concentration	1mg/ml
purification	Using SulfoLink™ Coupling Resin, the antiserum underwent purification through peptide affinity chromatography. The resulting product was highly purified and ready for use in future experiments.
Clonality	Polyclonal Antibody
Stability & Storage	The recommended storage condition for one year is at -20 °C. It is important to avoid subjecting the product to repeated freeze/thaw cycles.
Storage buffer	This sample contains Rabbit IgG in a solution of phosphate buffered saline with a pH of 7.4, along with 150mM NaCl, 0.02% sodium azide, and 50% glycerol. To maintain stability, it should be stored at a temperature of -20°C and will remain viable for 12 months from the date of receipt.
Target
Background	This oxidase enzyme exhibits a broad range of substrate specificity, allowing it to efficiently oxidize various aromatic azaheterocycles such as N1-methylnicotinamide, N-methylphthalazinium, and phthalazine. Additionally, it also catalyzes the oxidation of aldehydes like benzaldehyde, retinal, pyridoxal, and vanillin. Its crucial role lies in the metabolism of xenobiotics and drugs that contain aromatic azaheterocyclic substituents. Moreover, it plays a vital role in the activation of prodrugs, including famciclovir, by facilitating the oxidation process from 6-deoxypenciclovir to penciclovir, a highly potent antiviral agent. Another significant function of this enzyme is its involvement in maintaining the balance of reactive oxygen species within the body. Interestingly, it may serve as a prominent source of superoxide generation by one-electron reduction of molecular oxygen. Furthermore, it is capable of catalyzing the production of nitric oxide (NO) through the reduction of nitrite to NO using NADH or aldehyde as an electron donor. Finally, there is evidence suggesting its potential role in adipogenesis, the process of fat cell formation.
Tissue specificity	Abundant in liver, expressed in adipose tissue and at lower levels in lung, skeletal muscle, pancreas. In contrast to mice, no significant gender difference in AOX1 expression level (at protein level).
Celluar localization	Cytosol;Extracellular region or secreted;Nucleus;
UniPort	Q06278