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Rabbit Anti-AP2S1 Polyclonal Antibody#abs137360

Description

Catalog-specification	Delivery time	USD price
abs137360-100ug	1-2 Weeks	301.0
abs137360-50ug	1-2 Weeks	201.0

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Overview
catalog	abs137360
Other names	The sigma subunit of the adaptor protein complex 2, also known as AP2, plays a crucial role in clathrin-mediated endocytosis. It is encoded by the AP2S1 gene and is involved in the formation of clathrin-coated pits and vesicles. The AP2 complex, consisting of multiple subunits, helps in the selection and packaging of cargo proteins into vesicles for internalization. The sigma subunit, specifically sigma-2 adaptin, contributes to the stability and functioning of the complex. It is also referred to as AP17 or clathrin coat-associated protein AP17. This 17 kDa protein is localized to the plasma membrane and aids in the internalization of various molecules, enabling essential cellular processes.
Source	Rabbit
Specificity	The AP2S1 Antibody is capable of identifying total AP2S1 that exists naturally within an organism. A closely related content can be produced by altering the original text details while maintaining the same meaning.
Species Reactivity	Human
Predictive reaction species	Xenopus;Zebrafish;Chicken;Pig;Dog;Horse;Bovine;Sheep;
Antigen	AP2S1
Application	WB 1:1000-3000, ELISA(peptide) 1:20000-1:40000
Immunogen	A synthesized peptide derived from human AP2S1.
MW	17 kDa
Properties
Concentration	1mg/ml
purification	The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein Transport via Transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 alpha and AP-2 sigma subunits are thought to contribute to the recognition of the [ED]-X-X-X-L-[LI] motif (By similarity). May also play a role in extracellular calcium homeostasis.
Celluar localization	Cytosol;Endosome;Lysosome;Plasma Membrane;
UniPort	P53680