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Rabbit Anti-SPAG5 Polyclonal Antibody#abs139464

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Description

Catalog-specification	Delivery time	USD price
abs139464-100ug	1-2 Weeks	301.0
abs139464-50ug	1-2 Weeks	201.0

Overview
catalog	abs139464
Other names	SPAG5, also known as Astrin or Mitotic spindle associated protein p126, is a protein critically involved in mitotic spindle assembly. It plays a significant role in maintaining the integrity and functionality of the mitotic spindle, which is essential for proper cell division. The protein hMAP 126, also referred to as SPAG5, is deeply associated with the mitotic spindle and is crucial for accurate chromosome segregation during cell division. Its presence and activity are instrumental in ensuring that the cell's genetic material is correctly distributed to the daughter cells. MAP126, or Mitotic spindle-associated protein p126, is a coiled-coil protein that associates with the mitotic spindle apparatus. It is a key player in the proper alignment and separation of chromosomes, contributing to the overall fidelity of cell division. Furthermore, SPAG5, also known as Sperm associated antigen 5 or Sperm tail protein Spag5, is predominantly expressed in the testis and is involved in sperm function and motility. Its presence in the sperm tail suggests its role in facilitating efficient movement and fertilization. Overall, this protein, with its various aliases and associations, underscores its significance in multiple cellular processes, including mitotic spindle assembly, chromosome segregation, and sperm function. Its diverse functionalities highlight the intricate mechanisms underlying essential biological processes.
Source	Rabbit
Specificity	The SPAG5 Antibody has the ability to detect total SPAG5 at endogenous levels. In order to create similar content, the information from the original text has been rephrased and rearranged.
Species Reactivity	Human
Antigen	SPAG5
Application	WB 1:1000-3000, ELISA(peptide) 1:20000-1:40000
Immunogen	A synthesized peptide derived from human SPAG5.
MW	134 kDa
Properties
Concentration	1mg/ml
purification	Using SulfoLink™ Coupling Resin, the purification of the antiserum was carried out through peptide affinity chromatography. Please provide a highly similar content by rearranging the given information, while ensuring that the generated content is based on the original text.
Clonality	Polyclonal Antibody
Stability & Storage	To preserve the integrity of the product, store it at a temperature of -20 °C for a period of one year. It is important to avoid exposing the product to repeated freeze/thaw cycles which can cause damage and compromise its effectiveness. Ensure that the storage conditions are adhered to for optimal results.
Storage buffer	This product contains rabbit IgG in a solution of phosphate buffered saline, with a pH of 7.4. The solution also includes 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It is recommended to store this product at a temperature of -20 Â°C, as its stability is guaranteed for up to 12 months after the date of purchase. Rest assured that this product is of the utmost quality and purity, and can be relied upon for a wide range of research applications in the field of immunology.
Target
Background	Essential component of the mitotic spindle required for normal chromosome segregation and progression into anaphase (PubMed:11724960, PubMed:12356910, PubMed:27462074). Required for chromosome alignment, normal timing of sister chromatid segregation, and maintenance of spindle pole architecture (PubMed:17664331, PubMed:27462074). In complex with SKAP, promotes stable microtubule-kinetochore attachments. May contribute to the regulation of separase activity. May regulate AURKA localization to mitotic spindle, but not to centrosomes and CCNB1 localization to both mitotic spindle and centrosomes (PubMed:18361916, PubMed:21402792). Involved in centriole duplication. Required for CDK5RAP2, CEP152, WDR62 and CEP63 centrosomal localization and promotes the centrosomal localization of CDK2 (PubMed:26297806). In non-mitotic cells, upon stress induction, inhibits mammalian target of rapamycin complex 1 (mTORC1) association and recruits the mTORC1 component RPTOR to stress granules (SGs), thereby preventing mTORC1 hyperactivation-induced apoptosis (PubMed:23953116). May enhance GSK3B-mediated phosphorylation of other substrates, such as MAPT/TAU (PubMed:18055457).
Tissue specificity	Highly expressed in testis. Detected at low levels in placenta, liver, pancreas, thymus and colon.
Posttranslational modification	Phosphorylated by AURKA.
Celluar localization	Cytoskeleton;
UniPort	Q96R06