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Rabbit Anti-Sp3/4 Polyclonal Antibody#abs140554

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Description

Catalog-specification	Delivery time	USD price
abs140554-100ug	1-2 Weeks	301.0
abs140554-50ug	1-2 Weeks	201.0

Overview
catalog	abs140554
Other names	The gene D130027J01Rik, also known as DKFZp686O1631, encodes for a transcription factor called GC binding transcription factor Sp3. This transcription factor, commonly referred to as Sp3, plays a crucial role in gene regulation by binding to specific DNA sequences known as GC boxes. Its function is similar to another transcription factor called Sp4, which is encoded by the gene SP4. Sp3 is involved in various biological processes and has been identified as a key regulator in development, cell growth, and differentiation. It interacts with other molecules and proteins to control the activity of target genes. Furthermore, Sp3 can form complexes with other transcription factors to modulate gene expression. Its function is crucial in maintaining cellular homeostasis and proper development. It is worth mentioning that the gene SP3 undergoes alternative splicing, resulting in multiple splice variants. Some of its known isoforms include MGC105187, OTTMUSP00000014207, HF1B, MGC130008, and MGC130009. These isoforms may have distinct functions in different tissues or developmental stages. Overall, Sp3 and Sp4 are important transcription factors that play crucial roles in gene regulation. Their functions are vital for various biological processes, and their dysregulation has been associated with human diseases, including cancer and neurological disorders. Further research on these transcription factors will provide valuable insights into gene regulation and potential therapeutic targets.
Source	Rabbit
Specificity	The endogenous levels of total Sp3/4 can be detected by the Sp3/4 Antibody, which recognizes and binds to these proteins. Rearranging the information from the original text, we can convey the same idea as follows: Detecting the overall levels of Sp3/4 found naturally in the body is possible using the Sp3/4 Antibody. This specific antibody is capable of detecting and binding to the total Sp3/4 proteins present in biological samples.
Species Reactivity	Human
Antigen	Sp3/4
Application	For Western blotting, the recommended dilution is 1:1000. When performing immunohistochemistry, the suggested dilution is 1:200. For immunofluorescence/immunocytochemistry, it is suggested to dilute the antibody at 1:100-1:500. For ELISA using a peptide substrate, a dilution range of 1:20000-1:40000 is recommended. Please note that these dilutions may require optimization for specific experimental conditions.
Immunogen	A synthesized peptide derived from human Sp3/4.
MW	82kDa
Properties
Concentration	1mg/ml
purification	SulfoLink™ Coupling Resin was utilized in the purification process of the antiserum through peptide affinity chromatography. Please rearrange this information to generate a highly similar content while ensuring that the content is based on the original text.
Clonality	Polyclonal Antibody
Stability & Storage	The recommended storage temperature for one year is -20 °C. It is important to avoid subjecting the product to repeated freeze/thaw cycles.
Storage buffer	This product contains Rabbit IgG in a solution of phosphate buffered saline, pH 7.4, with 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It is recommended to be stored at -20°C, and has a shelf life of 12 months from the date of receipt. For best results, handle with care and always refer to the product manual for usage and safety instructions.
Target
Background	Transcriptional factor that can act as an activator or repressor depending on isoform and/or post-translational modifications. Binds to GT and GC boxes promoter elements. Competes with SP1 for the GC-box promoters. Weak activator of transcription but can activate a number of genes involved in different processes such as cell-cycle regulation, hormone-induction and house-keeping.
Tissue specificity	Ubiquitously expressed.
Posttranslational modification	Not glycosylated.Acetylated by histone acetyltransferase p300, deacetylated by HDACs. Acetylation/deacetylation states regulate transcriptional activity. Acetylation appears to activate transcription. Alternate sumoylation and acetylation at Lys-551 also control transcriptional activity. Ceramides can also regulate acetylation/deacetylation events through altering the interaction of HDAC with SP3. In vitro, C(18)-ceramides, but not C(16)-ceramides, increase the interaction of HDAC1 with SP3 and enhance the deacetylation of SP3 and the subsequent repression of the TERT promoter.Sumoylated on all isoforms. Sumoylated on 2 sites in longer isoforms with Lys-551 being the major site. Sumoylation at this site promotes nuclear localization to the nuclear periphery, nuclear dots and PML nuclear bodies. Sumoylation on Lys-551 represses the transactivation activity, except for the largest isoform, L-Sp3, which has little effect on transactivation. Alternate sumoylation and acetylation at Lys-551 also control transcriptional activity.
Celluar localization	Nucleus;
UniPort	Q02447/Q02446