Rabbit Anti-MMP1 Polyclonal Antibody #abs130146

abs130146-50ug

1-2 Weeks

201

abs130146-100ug

1-2 Weeks

301

Description

MMP1 is a member of the peptidase M10A family and it plays a crucial role in collagen degradation. Specifically, it cleaves collagens of types I, II, and III at a single site within the helical domain. Additionally, MMP1 is capable of cleaving collagens of types VII and X. Interestingly, in the context of HIV infection, MMP1 also interacts with and cleaves the secreted viral Tat protein. This interaction with Tat leads to a reduction in the neurotoxic effects mediated by neuronal Tat. Overall, MMP1's diverse cleavage abilities and its involvement in mitigating Tat-induced neurotoxicity highlight its significance in both collagen metabolism and HIV-related neuroprotection.

Other names

Collagenase, also known as interstitial collagenase, is a protein enzyme with a molecular weight of 27 kDa. It is referred to by various names including CLG, CLGN, fibroblast collagenase, and matrix metallopeptidase 1 (interstitial collagenase). Other aliases for this enzyme include matrix metalloprotease 1, matrix metalloproteinase-1, MMP 1, MMP-1, MMP1, MMP1_HUMAN, and OTTHUMP00000045866. This collagenase plays a crucial role in the breakdown of collagen in the extracellular matrix and is primarily found in fibroblast cells. By rearranging and reorganizing the provided information, I have generated a highly similar content while retaining the original text's information.

Source

Rabbit

Specificity

The MMP1 antibody is capable of detecting the complete levels of MMP1 protein that naturally occur within an organism. This antibody is specifically designed to recognize and bind to MMP1, which enables researchers to visualize and measure the expression of this protein. By using the MMP1 antibody, researchers can gain a better understanding of the role that MMP1 plays in various biological processes. Furthermore, this antibody is a valuable tool for investigating the potential therapeutic applications of inhibitors that target MMP1.

Species Reactivity

Human

Predictive reaction species

Rabbit;Horse;

Application

The recommended dilution for Western blotting is between 1:500 to 1:3000, while for IF/ICC it is 1:100 to 1:500. For ELISA using a peptide, a dilution of 1:20000 to 1:40000 is suggested. It's important to note that these are just general guidelines and may need to be optimized for individual experiments. Therefore, it's important to perform a titration experiment to determine the optimal dilution for your specific application.

Immunogen

A synthesized peptide derived from human MMP1.

Properties

Concentration

1mg/ml

Purification

SulfoLink™ Coupling Resin was used for peptide affinity chromatography to purify the antiserum.

Clonality

Polyclonal Antibody

Stability & Storage

To ensure optimal storage, it is recommended that this product be kept at a temperature of -20 °C for a duration of one year. It is important to avoid subjecting the product to repeated cycles of freezing and thawing, which can have detrimental effects.

Storage buffer

The storage of Rabbit IgG involves keeping it in a solution of phosphate buffered saline, with a pH of 7.4. The solution also contains 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It is important to store it at a temperature of -20 °C. The stability of the IgG is guaranteed for a period of 12 months, starting from the date of receipt.

Target

Background

This enzyme is known for its ability to cleave various types of collagens, including types I, II, III, VII, and X. It targets a specific site within the helical domain of these collagens, and its cleavage activity has been extensively studied and documented (PubMed:2557822, PubMed:2153297, PubMed:1645757).
Interestingly, this enzyme has been found to interact with and cleave the secreted Tat protein of the HIV virus. This interaction results in a reduction of Tat-mediated neurotoxicity in neuronal cells (PubMed:16807369).
It's worth noting that the enzymatic activity of this protein is crucial in multiple biological contexts, where collagen breakdown or regulation is required. Additionally, its potential involvement in HIV-related neurotoxicity highlights its significance in viral pathogenesis and the potential for therapeutic intervention.

Posttranslational modification

Undergoes autolytic cleavage to two major forms (22 kDa and 27 kDa). A minor form (25 kDa) is the glycosylated form of the 22 kDa form. The 27 kDa form has no activity while the 22/25 kDa form can act as activator for collagenase.Tyrosine phosphorylated in platelets by PKDCC/VLK.

Celluar localization

Extracellular region or secreted;

UniPort

P03956