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Rabbit Anti-Smurf1 Polyclonal Antibody#abs137216

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Description

Catalog-specification	Delivery time	USD price
abs137216-100ug	1-2 Weeks	301.0
abs137216-50ug	1-2 Weeks	201.0

Overview
catalog	abs137216
Other names	Smurf1, also known as E3 ubiquitin-protein ligase SMURF1 or hSMURF1, is a protein that plays a critical role in the regulation of cellular processes. It is encoded by the KIAA1625 gene and is involved in the ubiquitination of Smad proteins. Smurf1 acts as a specific E3 ubiquitin ligase for Smad proteins, targeting them for degradation. The function of Smurf1 is to control the activity and stability of Smad proteins, which are key regulators of various signaling pathways, including the transforming growth factor-beta (TGF-β) pathway. Smad proteins transduce signals from the cell membrane to the nucleus, where they modulate gene expression and regulate cell growth, differentiation, and other cellular processes. Through its E3 ligase activity, Smurf1 binds to Smad proteins and adds ubiquitin molecules to them, marking them for degradation by the proteasome. This ubiquitination process regulates the availability and activity of Smad proteins, thus controlling the duration and intensity of TGF-β signaling. Smurf1 has also been implicated in the regulation of other signaling pathways and cellular processes. It has been shown to modulate the stability of various proteins involved in cell cycle regulation, cell migration, and cytoskeletal organization. In conclusion, Smurf1 is an important regulator of cellular processes through its role as a specific E3 ubiquitin ligase for Smad proteins. It is involved in the control of TGF-β signaling and other cellular pathways, highlighting its significance in various physiological and pathological contexts.
Source	Rabbit
Specificity	Detection of endogenous levels of total Smurf1 is possible using the Smurf1 Antibody. Additionally, it is important to note that the content should be a highly similar rearrangement of the original text rather than creating entirely different content using a language model.
Species Reactivity	Human;Mouse
Antigen	Smurf1
Application	WB 1:1000-3000, ELISA(peptide) 1:20000-1:40000
Immunogen	A synthesized peptide derived from human Smurf1.
MW	86 kDa
Properties
Concentration	1mg/ml
purification	Peptide affinity chromatography utilizing SulfoLink™ Coupling Resin was employed in the purification of the antiserum.
Clonality	Polyclonal Antibody
Stability & Storage	To maintain the quality of the product, it is recommended to store it at a temperature of -20 °C for a period of one year. To prevent any damage, it is advised to avoid exposing the product to repeated freeze and thaw cycles.
Storage buffer	The Rabbit IgG solution comes in a phosphate buffered saline with a pH of 7.4, 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It is recommended to store the solution at -20 °C, and it remains stable for up to 12 months from the date of receipt.
Target
Background	E3 ubiquitin-protein ligase that acts as a negative regulator of BMP signaling pathway. Mediates ubiquitination and degradation of SMAD1 and SMAD5, 2 receptor-regulated SMADs specific for the BMP pathway. Promotes ubiquitination and subsequent proteasomal degradation of TRAF family members and RHOA. Promotes ubiquitination and subsequent proteasomal degradation of MAVS (PubMed:23087404). Plays a role in dendrite formation by melanocytes (PubMed:23999003).
Tissue specificity	Expressed in melanocytes (PubMed:23999003).
Posttranslational modification	Auto-ubiquitinated in presence of NDFIP1 (PubMed:23087404). Ubiquitinated by the SCF(FBXL15) complex at Lys-381 and Lys-383, leading to its degradation by the proteasome. Lys-383 is the primary ubiquitination site.
Celluar localization	Cytosol;Extracellular region or secreted;Nucleus;Plasma Membrane;
UniPort	Q9HCE7