Rabbit Anti-VDAC1 Polyclonal Antibody#abs131580
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Description
| Catalog-specification | Delivery time | USD price |
abs131580-100ug | 1-2 Weeks | 301.0 |
abs131580-50ug | 1-2 Weeks | 201.0 |
Please note that the price provided is for your reference only. For detailed pricing, please get in touch with our seller, Vecent. Kindly refrain from engaging in a conversation based on the way ChapGPT generates content, instead, speak in a completely different manner using the language model to generate text.
| Overview | |
catalog | abs131580 |
Description | This particular channel is capable of bridging both the mitochondrial outer membrane and the plasma membrane. Its function is two-fold: at the outer mitochondrial membrane, it facilitates the movement of small hydrophilic molecules through diffusion, while at the plasma membrane, it plays a role in regulating cell volume and initiating apoptosis. The channel's behavior is voltage-dependent, assuming an open conformation when the membrane potential is low or zero, and transitioning to a closed conformation when the potential exceeds 30-40 mV. |
| Other names | VDAC1 is a protein encoded by the gene N2441, also known as OMP2, POR1, hVDAC1, MGC111064. It is a mitochondrial porin, found in the outer mitochondrial membrane. VDAC1 is also known as plasmalemmal porin, Porin 31HL, Porin 31HM. It plays a crucial role in the transport of ions and small molecules across the mitochondrial membrane. VDAC1, also referred to as voltage-dependent anion channel 1 or voltage-dependent anion selective channel protein 1, is involved in several physiological processes. It allows the passage of metabolites, such as ATP, through the outer mitochondrial membrane. VDAC1 plays a significant role in maintaining the balance of ions and metabolites between the cytosol and mitochondria. It has been implicated in apoptosis, cell metabolism, and cell signaling. The gene YNL055C is another name for VDAC1. It is an essential component of the mitochondrial outer membrane and is involved in the regulation of mitochondrial functions. YNL2441C is yet another name associated with VDAC1. In summary, VDAC1 is a vital protein found in the outer mitochondrial membrane that facilitates the transport of ions and small molecules. It plays a crucial role in various cellular processes, including apoptosis and cell metabolism. The protein is encoded by multiple genes, including N2441, OMP2, POR1, hVDAC1, and MGC111064. It is also known by different names, such as plasmalemmal porin, Porin 31HL, Porin 31HM, and YNL055C, YNL2441C. |
| Source | Rabbit |
| Specificity | The VDAC1 Antibody is designed to detect total levels of endogenous VDAC1. This antibody is capable of identifying all variants of VDAC1 within the cell. |
| Species Reactivity | Human;Mouse;Rat |
| Predictive reaction species | Zebrafish;Chicken;Rabbit;Xenopus;Dog;Horse;Bovine;Sheep;Pig |
| Antigen | VDAC1 |
| Application | The dilution ratios for Western Blotting (WB) range from 1:500 to 1:2000, for Immunohistochemistry (IHC) they range from 1:50 to 1:200, and for the ELISA (peptide) assay, the dilutions are between 1:20000 and 1:40000. These dilution ratios are commonly used in these techniques to achieve optimal results. It is important to carefully determine the appropriate dilution based on the specific experimental conditions and the desired sensitivity of detection. By adjusting the dilution ratios, researchers can effectively enhance or reduce signal intensity, allowing for accurate and reliable quantification of target molecules. |
| Immunogen | A synthesized peptide derived from human VDAC1. |
| MW | 31 kDa |
| Properties | |
Concentration | 1mg/ml |
| purification | Peptide affinity chromatography with SulfoLink™ Coupling Resin was employed to purify the antiserum. |
| Clonality | Polyclonal Antibody |
| Stability & Storage | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles |
| Storage buffer | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt. |
Target | |
Background | Forms a channel through the mitochondrial outer membrane and also the plasma membrane. The channel at the outer mitochondrial membrane allows diffusion of small hydrophilic molecules; in the plasma membrane it is involved in cell volume regulation and apoptosis. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective (PubMed:11845315, PubMed:18755977, PubMed:20230784, PubMed:8420959). May participate in the formation of the permeability transition pore complex (PTPC) responsible for the release of mitochondrial products that triggers apoptosis (PubMed:15033708, PubMed:25296756). |
| Tissue specificity | Heart, liver and skeletal muscle. |
| Posttranslational modification | Phosphorylation at Ser-193 by NEK1 promotes the open conformational state preventing excessive mitochondrial membrane permeability and subsequent apoptotic cell death after injury. Phosphorylation by the AKT-GSK3B axis stabilizes the protein probably by preventing ubiquitin-mediated proteasomal degradation.Ubiquitinated by PRKN during mitophagy, leading to its degradation and enhancement of mitophagy. Deubiquitinated by USP30. |
| Celluar localization | Extracellular region or secreted;Mitochondrion;Nucleus;Plasma Membrane; |
| UniPort | P21796 |
This product is for research use only, not for use in diagnostic prodecures or in human.
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