Rabbit Anti-TBP Polyclonal Antibody#abs131578
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Description
| Catalog-specification | Delivery time | USD price |
abs131578-50ug | 1-2 Weeks | 201.0 |
abs131578-100ug | 1-2 Weeks | 301.0 |
Please note that the price provided is for your reference only. For detailed pricing information, please reach out to our seller, Vecent. Kindly refrain from using ChapGPT to generate similar content, as it is important to communicate in a distinctly different manner using language model-generated text.
| Overview | |
catalog | abs131578 |
Description | TFIID is a crucial component of the DNA-binding multiprotein factor that is involved in transcription. It acts as a general transcription factor and is responsible for the initial step of transcription, which is the binding of TFIID to the TATA box. This process forms the pre-initiation complex (PIC) and plays a crucial role in the activation of genes in eukaryotes that are transcribed by RNA polymerase II. |
| Other names | TBP, also known as TATA-binding factor or TATA box factor, is a crucial component of the transcription initiation factor TFIID. It binds to the TATA sequence in DNA and plays a significant role in the regulation of gene expression. TBP has different isoforms, including GTF2D, GTF2D1, HDL4, MGC117320, MGC126054, MGC126055, and SCA17. The TBP protein contains a TATA-box binding protein N-terminal domain, which is responsible for its interaction with other transcription factors. Its presence in TFIID ensures the formation of the pre-initiation complex necessary for transcription initiation. TBP acts as a TATA sequence binding protein, recognizing the TATA box motif in the promoter region of genes. Its binding to the TATA sequence is essential for the recruitment of other components involved in transcription, such as RNA polymerase II and general transcription factors. The TBP subunit of TFIID is vital for the accurate and efficient initiation of transcription. It provides a platform for the assembly of the pre-initiation complex and helps in positioning RNA polymerase II at the transcription start site. Through its interaction with various transcription factors, TBP regulates gene expression and controls cellular processes. In summary, TBP, also known as TATA-binding factor or TATA box factor, is a critical component of the transcription initiation factor TFIID. It binds to the TATA sequence in DNA and plays a vital role in gene expression regulation. Its diverse isoforms, including GTF2D, GTF2D1, HDL4, MGC117320, MGC126054, MGC126055, and SCA17, ensure its functional versatility. TBP's interaction with other transcription factors and its TATA-box binding protein N-terminal domain contribute to the accurate initiation of transcription. |
| Source | Rabbit |
| Specificity | The TBP Antibody is capable of detecting the total levels of TBP that naturally occur in the body. To be more specific, this antibody is designed to recognize and bind to the endogenous TBP protein. Its purpose is to enable the measurement and analysis of the overall amount of TBP present in a biological system. This detection method provides valuable insights into the understanding of TBP function and regulation. |
| Species Reactivity | Human;Mouse;Rat |
| Predictive reaction species | Chicken;Pig;Dog;Horse;Bovine |
| Antigen | TBP |
| Application | The dilution factors for Western Blot (WB) range from 1:500 to 1:2000. For Immunohistochemistry (IHC), the dilution factors are between 1:50 and 1:200. As for Immunofluorescence/Immunocytochemistry (IF/ICC), the recommended dilution range is 1:100 to 1:500. In the case of ELISA assays using peptides, the dilution factors should be around 1:20000 to 1:40000. Please note that this content has been rearranged to maintain accuracy while presenting the information in a different manner. |
| Immunogen | A synthesized peptide derived from human TBP. |
| MW | 38 kDa |
| Properties | |
Concentration | 1mg/ml |
| purification | Using SulfoLink™ Coupling Resin, the antiserum was subjected to peptide affinity chromatography to achieve purification. |
| Clonality | Polyclonal Antibody |
| Stability & Storage | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles |
| Storage buffer | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt. |
Target | |
Background | General transcription factor that functions at the core of the DNA-binding multiprotein factor TFIID (PubMed:2374612, PubMed:2363050, PubMed:2194289, PubMed:9836642, PubMed:27193682). Binding of TFIID to the TATA box is the initial transcriptional step of the pre-initiation complex (PIC), playing a role in the activation of eukaryotic genes transcribed by RNA polymerase II (PubMed:2374612, PubMed:2363050, PubMed:2194289, PubMed:9836642, PubMed:27193682). Component of a BRF2-containing transcription factor complex that regulates transcription mediated by RNA polymerase III (PubMed:26638071). Component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC (pre-initiation complex) during RNA polymerase I-dependent transcription (PubMed:15970593). The rate of PIC formation probably is primarily dependent on the rate of association of SL1 with the rDNA promoter. SL1 is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA. |
| Tissue specificity | Widely expressed, with levels highest in the testis and ovary. |
| Celluar localization | Nucleus; |
| UniPort | P20226 |
This product is for research use only, not for use in diagnostic prodecures or in human.
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