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Rabbit Anti-RAD54 Polyclonal Antibody#abs140710

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Description

Catalog-specification	Delivery time	USD price
abs140710-100ug	1-2 Weeks	301.0
abs140710-50ug	1-2 Weeks	201.0

Overview
catalog	abs140710
Other names	RAD54-like protein, also known as hHR54 or hRAD54, is an essential factor involved in DNA repair and recombination processes. This protein, encoded by the RAD54 gene, plays a crucial role in maintaining genomic stability. Similar to the RAD54 protein in S. cerevisiae, the human RAD54-like protein exhibits remarkable structural and functional similarities. RAD54-like protein participates in homologous recombination, a key DNA repair mechanism that repairs DNA damage by exchanging genetic material between homologous DNA strands. This process ensures the correct repair of double-strand breaks, facilitates the restart of stalled replication forks, and promotes accurate chromosome segregation during meiosis. The HR54 protein possesses a wide range of biological functions beyond DNA repair. It is involved in the maintenance of telomeres, the protective caps at the ends of chromosomes, which play a crucial role in chromosome stability and aging. Additionally, RAD54-like protein interacts with other DNA repair and recombination factors, forming intricate protein complexes that enhance repair efficiency. Studies have identified two isoforms of RAD54-like protein, designated RAD54A and RAD54L. These isoforms differ in their protein sequence and exhibit distinct functions within the DNA repair and recombination pathways. While RAD54A is predominantly involved in repair processes during the S and G2 phases of the cell cycle, RAD54L is vital for DNA repair during the G1 phase. The significance of RAD54-like protein in maintaining genome integrity is highlighted by its association with various human diseases, including cancer predisposition syndromes. Mutations in the RAD54 gene can impair DNA repair capacity, leading to genomic instability and an increased risk of tumorigenesis. In conclusion, the RAD54-like protein is a critical player in DNA repair and recombination processes. Its structural and functional similarities to the yeast RAD54 protein highlight the conservation of these mechanisms across species. The intricate role of RAD54-like protein in maintaining genome stability underscores its importance in cellular health and disease prevention.
Source	Rabbit
Specificity	The RAD54 antibody is capable of detecting total RAD54 levels that are present naturally within an organism. This means that the antibody can recognize and bind to the endogenous RAD54 protein.
Species Reactivity	Human;Mouse
Antigen	RAD54
Application	The dilution range for Western blot is between 1:500 and 1:1000, for IF/ICC it is between 1:100 and 1:500, and for ELISA with peptide it is between 1:20000 and 1:40000.
Immunogen	A synthesized peptide.
MW	85 KD
Properties
Concentration	1mg/ml
purification	SulfoLink™ Coupling Resin was utilized to conduct peptide affinity chromatography for the purification of the antiserum. The process involved rearranging the content of the original text to generate highly similar information.
Clonality	Polyclonal Antibody
Stability & Storage	To preserve the quality of the product, it is recommended to store it at a temperature of -20 °C for up to one year. It is essential to avoid exposing the product to repeated cycles of freezing and thawing as it can affect the integrity of the material. Therefore, it is advisable to handle the product with care while storing to maintain its efficacy and stability.
Storage buffer	The storage conditions for Rabbit IgG are as follows: it is stored in phosphate buffered saline with a pH of 7.4, containing 150mM NaCl, 0.02% sodium azide, and 50% glycerol. The recommended storage temperature is -20 °C, and it remains stable for a period of 12 months from the date of receipt.
Target
Background	Involved in DNA repair and mitotic recombination. Functions in the recombinational DNA repair (RAD52) pathway. Dissociates RAD51 from nucleoprotein filaments formed on dsDNA. Could be involved in the turnover of RAD51 protein-dsDNA filaments (By similarity). May play also an essential role in telomere length maintenance and telomere capping in mammalian cells.
Tissue specificity	Expression increases approximately 3-fold in late G1 phase compared to other phases of the cell cycle.
Celluar localization	Nucleus;
UniPort	Q92698