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Rabbit Anti-RAD51L3 Polyclonal Antibody#abs140709

Description

Catalog-specification	Delivery time	USD price
abs140709-100ug	1-2 Weeks	301.0
abs140709-50ug	1-2 Weeks	201.0

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Overview
catalog	abs140709
Other names	RAD51 homolog 4, also known as BROVCA4 or DNA repair protein, is a crucial component involved in the repair of damaged DNA. It is highly similar to the RAD51 homolog D found in the yeast Saccharomyces cerevisiae. The human counterpart, known as HsTRAD or RAD51-like protein 3, plays a vital role in recombination repair. The gene encoding this protein is known by various names, including OTTHUMP00000163851, OTTHUMP00000163852, and OTTHUMP00000163853. It is also referred to as R51H3, RA51D_HUMAN, or RAD51 paralog D. The protein is responsible for promoting accurate repair of DNA strands, ensuring genomic stability. The importance of RAD51 homolog 4 in DNA repair has been extensively studied. It shares functional similarities with the yeast RAD51 homolog D and plays a critical role in maintaining genome integrity. The protein's activity is crucial in repairing damaged DNA and preventing the accumulation of harmful mutations. Understanding the functions and mechanisms of RAD51 homolog 4 can provide valuable insights into the repair processes of damaged DNA. Further research on this protein may pave the way for the development of novel therapeutic strategies targeting DNA repair pathways, particularly in the context of cancer treatment.
Source	Rabbit
Specificity	The total RAD51L3 levels can be detected using the RAD51L3 Antibody, which specifically targets the endogenous RAD51L3 protein. By utilizing this antibody, it becomes possible to accurately measure the natural expression of RAD51L3 within an organism.
Species Reactivity	Human
Antigen	RAD51L3
Application	For western blotting, the recommended dilutions are between 1:500 and 1:1000. Meanwhile, for immunofluorescence and immunocytochemistry, dilutions of 1:100 to 1:500 are advised. For ELISA using peptides, a dilution range of 1:20000 to 1:40000 is appropriate. It is important to note that these dilutions are based on the original information provided and are essential for achieving accurate results.
Immunogen	A synthesized peptide.
MW	40 KD
Properties
Concentration	1mg/ml
purification	By employing SulfoLink™ Coupling Resin, the antiserum underwent purification through peptide affinity chromatography. It was subjected to a process where the original antigens were immobilized and selectively bound to the resin, allowing for effective separation and purification of the antiserum.
Clonality	Polyclonal Antibody
Stability & Storage	To preserve the quality of the product, it is recommended to store it at a temperature of -20 °C for up to one year. It is important to avoid exposing the product to repeated freezing and thawing cycles as this may compromise its integrity. Taking these precautions will help ensure the product remains usable and effective for its intended purpose.
Storage buffer	The Rabbit IgG comes in a solution of phosphate buffered saline with a pH of 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. It is recommended to store the product at a temperature of -20 °C to maintain its stability. The solution can be stored safely for up to 12 months from the date of receipt.
Target
Background	Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA breaks arising during DNA replication or induced by DNA-damaging agents. Bind to single-stranded DNA (ssDNA) and has DNA-dependent ATPase activity. Part of the Rad21 paralog protein complex BCDX2 which acts in the BRCA1-BRCA2-dependent HR pathway. Upon DNA damage, BCDX2 acts downstream of BRCA2 recruitment and upstream of RAD51 recruitment. BCDX2 binds predominantly to the intersection of the four duplex arms of the Holliday junction and to junction of replication forks. The BCDX2 complex was originally reported to bind single-stranded DNA, single-stranded gaps in duplex DNA and specifically to nicks in duplex DNA. Involved in telomere maintenance. The BCDX2 subcomplex XRCC2:RAD51D can stimulate Holliday junction resolution by BLM.
Tissue specificity	Expressed in colon, prostate, spleen, testis, ovary, thymus and small intestine. Weakly expressed in leukocytes.
Celluar localization	Cytoskeleton;Nucleus;
UniPort	O75771