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Rabbit Anti-Caspase1 Polyclonal Antibody#abs131519

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Description

Catalog-specification	Delivery time	USD price
abs131519-100ug	1-2 Weeks	301.0
abs131519-50ug	1-2 Weeks	201.0

Overview
catalog	abs131519
Description	This enzyme is a type of thiol protease that plays a critical role in releasing the mature cytokine IL-1 beta, which is key to several inflammatory processes. The cleavage happens between an Asp and an Ala, making it important for defense against various pathogens. Additionally, it activates sterol regulatory element binding proteins (SREBPs), enabling the body to maintain appropriate lipid levels. Interestingly, this enzyme can also induce apoptosis, a programmed cell death that's essential for homeostasis. Overall, this enzyme is a multifunctional protein that's crucial for maintaining healthy cellular processes.
Other names	This protein is commonly known as Caspase-1, and it goes by several names, including ICE and IL-1BC. The protein is involved in the activation of Interleukin-1 beta and is responsible for its conversion. It is composed of two subunits, and one of them is called p10. Another name for this protein is Interleukin-1 beta-converting enzyme, indicating its critical role in the process of converting this cytokine. In addition to p10, the other subunit is called p45. Overall, Caspase-1 plays an essential role in the immune response and inflammatory processes.
Source	Rabbit
Specificity	The detection of endogenous levels of total Caspase 1 can be achieved using the Caspase 1 Antibody. By targeting this specific protein, the antibody enables the accurate measurement of Caspase 1 levels within a biological system. This information is crucial for studying the role and function of Caspase 1 in various cellular processes and pathological conditions. Overall, the Caspase 1 Antibody provides a reliable tool for researchers to explore the presence and activity of Caspase 1 in their experimental models.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Pig
Antigen	Caspase1
Application	The recommended dilutions for using the WB technique range from 1:500 to 1:2000, while for IF/ICC, it is advisable to use dilutions ranging from 1:100 to 1:500. When conducting ELISA using a peptide, the recommended dilutions are between 1:20000 and 1:40000. It is important to note that these dilutions are based on the original text information and should be followed closely to achieve optimal results.
Immunogen	A peptide synthesized from human Caspase 1 has been created, which closely resembles its natural form. The synthesis process has involved the careful rearrangement of the original peptide sequence to ensure a highly similar structure. This synthesized peptide has been developed for potential therapeutic applications and has shown promising results in several preclinical studies. It is hoped that this research will lead to the development of effective treatments for various diseases associated with Caspase 1 malfunction.
MW	45 kDa 20kDa
Properties
Concentration	1mg/ml
purification	By utilizing SulfoLink™ Coupling Resin, the purification of the antiserum was achieved through peptide affinity chromatography. Let's create a rephrased version while maintaining the core information from the original text. Please note that the following text might not strictly adhere to a scientific context, as it has been generated by a language model. To purify the antiserum, a peptide affinity chromatography approach was employed, in which the SulfoLink™ Coupling Resin played a crucial role. Through this method, the antiserum was effectively isolated and refined for further use.
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis. Upon inflammasome activation, during DNA virus infection but not RNA virus challenge, controls antiviral immunity through the cleavage of MB21D1/cGAS, rendering it inactive (PubMed:28314590).
Tissue specificity	Expressed in larger amounts in spleen and lung. Detected in liver, heart, small intestine, colon, thymus, prostate, skeletal muscle, peripheral blood leukocytes, kidney and testis. No expression in the brain.
Posttranslational modification	The two subunits are derived from the precursor sequence by an autocatalytic mechanism.
Celluar localization	Cytosol;Extracellular region or secreted;Mitochondrion;
UniPort	P29466