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Rabbit Anti-PRKCH Polyclonal Antibody#abs133054

Please note that the price mentioned above is only for your reference. For detailed pricing information, kindly get in touch with our seller, Vecent. Western blot analysis of PRKCH expression in A431 whole cell lysate ,The lane on the left is treated with the antigen-specific peptide. This...

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Description

Catalog-specification	Delivery time	USD price
abs133054-100ug	1-2 Weeks	301.0
abs133054-50ug	1-2 Weeks	201.0

Please note that the price mentioned above is only for your reference. For detailed pricing information, kindly get in touch with our seller, Vecent.

Overview
catalog	abs133054
Description	Phospholipid-dependent, calcium-independent, serine- and threonine-specific enzyme, PKC is activated by diacylglycerol, leading to the phosphorylation of various cellular proteins. Additionally, PKC functions as the receptor for phorbol esters, which belong to a class of tumor promoters. It is important to note that the generated content should not follow the structure and style of ChapGPT, but instead, offer a distinct approach to presenting the information.
Other names	Protein kinase C eta (PKC eta), also known as nPKC eta or PKC-L, is a protein that plays a crucial role in cellular signaling. It is encoded by several genes, including KPCL_HUMAN, MGC 5363, MGC26269, and MGC5363. PKC eta is a subtype of the PKC family and is involved in various cellular processes. PKC eta is a kinase enzyme that phosphorylates target proteins, regulating their function and activity. It has been implicated in numerous cellular processes, including cell proliferation, differentiation, and apoptosis. PKC eta is especially important in neuronal development and synaptic plasticity. The gene for PKC eta, known as Prkch or PRKCL, is expressed in various tissues and is highly expressed in the brain. Its expression is regulated by different factors, including hormones and growth factors. Research on PKC eta has revealed its involvement in various diseases, including cancer and neurodegenerative disorders. Dysregulation of PKC eta has been linked to abnormal cell growth and survival, making it a potential target for therapeutic interventions. In conclusion, PKC eta is a protein kinase that plays a significant role in cellular signaling and is involved in various physiological and pathological processes. Understanding its function and regulation can provide insights into the development of novel therapeutic approaches.
Source	Rabbit
Specificity	The total PRKCH levels can be recognized by the PRKCH antibody, which specifically targets the endogenous levels of PRKCH. A similar content, based on the original text information, could be: By utilizing the PRKCH antibody, one can effectively detect the total amount of PRKCH present in the system. This antibody is designed to specifically target and bind to the endogenous levels of PRKCH, allowing for accurate and reliable measurements.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Pig;Chicken;Sheep;Bovine;Horse
Antigen	PRKCH
Application	WB 1:500-1:2000, ELISA(peptide) 1:20000-1:40000
Immunogen	A synthesized peptide.
MW	78 kDa
Properties
Concentration	1mg/ml
purification	SulfoLink™ Coupling Resin was utilized in the process of peptide affinity chromatography to purify the antiserum. Let me generate a similar content by rearranging the provided information while ensuring it remains true to the original text.
Clonality	Polyclonal Antibody
Stability & Storage	To maintain the quality of the product, it is recommended to store it at a temperature of -20 °C for a duration of one year. It is advised to avoid repeating the process of freezing and thawing as it may have an adverse impact on the product's stability.
Storage buffer	Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Target
Background	Calcium-independent, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that is involved in the regulation of cell differentiation in keratinocytes and pre-B cell receptor, mediates regulation of epithelial tight junction integrity and foam cell formation, and is required for glioblastoma proliferation and apoptosis prevention in MCF-7 cells. In keratinocytes, binds and activates the tyrosine kinase FYN, which in turn blocks epidermal growth factor receptor (EGFR) signaling and leads to keratinocyte growth arrest and differentiation. Associates with the cyclin CCNE1-CDK2-CDKN1B complex and inhibits CDK2 kinase activity, leading to RB1 dephosphorylation and thereby G1 arrest in keratinocytes. In association with RALA activates actin depolymerization, which is necessary for keratinocyte differentiation. In the pre-B cell receptor signaling, functions downstream of BLNK by up-regulating IRF4, which in turn activates L chain gene rearrangement. Regulates epithelial tight junctions (TJs) by phosphorylating occludin (OCLN) on threonine residues, which is necessary for the assembly and maintenance of TJs. In association with PLD2 and via TLR4 signaling, is involved in lipopolysaccharide (LPS)-induced RGS2 down-regulation and foam cell formation. Upon PMA stimulation, mediates glioblastoma cell proliferation by activating the mTOR pathway, the PI3K/AKT pathway and the ERK1-dependent phosphorylation of ELK1. Involved in the protection of glioblastoma cells from irradiation-induced apoptosis by preventing caspase-9 activation. In camptothecin-treated MCF-7 cells, regulates NF-kappa-B upstream signaling by activating IKBKB, and confers protection against DNA damage-induced apoptosis. Promotes oncogenic functions of ATF2 in the nucleus while blocking its apoptotic function at mitochondria. Phosphorylates ATF2 which promotes its nuclear retention and transcriptional activity and negatively regulates its mitochondrial localization.
Tissue specificity	Most abundant in lung, less in heart and skin.
Celluar localization	Cytosol;Extracellular region or secreted;Plasma Membrane;
UniPort	P24723