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Rabbit Anti-NDFIP2 Polyclonal Antibody#abs132975

Please note that the price mentioned above is only for your reference. For detailed pricing information, please get in touch with our seller, Vecent. Western blot analysis of NDFIP2 expression in 721_B cell lysate ,The lane on the left is treated with the antigen-specific peptide. This product...

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Description

Catalog-specification	Delivery time	USD price
abs132975-100ug	1-2 Weeks	301.0
abs132975-50ug	1-2 Weeks	201.0

Please note that the price mentioned above is only for your reference. For detailed pricing information, please get in touch with our seller, Vecent.

Overview
catalog	abs132975
Description	ITCH, NEDD4, NEDD4L, SMURF2, WWP1, and WWP2 are E3 ubiquitin-protein ligases that contain the HECT domain and can be activated by it. These ligases play a crucial role in modulating the stability of their targets, thus controlling various cellular processes. One of the functions of this activation is recruiting ITCH, NEDD4, and SMURF2 to endosomal membranes. Another significant impact is the potential modulation of EGFR signaling. By rearranging the content, we can emphasize the activation of E3 ubiquitin-protein ligases through the HECT domain and how it influences their targets' stability, as well as the recruitment process and its potential impact on EGFR signaling.
Other names	Ndfip2, also known as NEDD4 family interacting protein 2 or NEDD4 WW domain binding protein 5A, is a putative MAPK activating protein PM04/PM05/PM06/PM07 and putative NF kappa B activating protein 413. It is a member of the NEDD4 family-interacting protein family and plays a role in various cellular processes, including the regulation of protein-protein interactions and ubiquitin-mediated protein degradation. Ndfip2 is known to interact with the WW domains of NEDD4 family members and may be involved in the regulation of their activity. Additionally, it has been suggested that Ndfip2 may activate MAPK and NF-kappa-B signaling pathways, although further research is needed to confirm this. Overall, Ndfip2 appears to be a multifunctional protein with diverse roles in cellular signaling and protein regulation.
Source	Rabbit
Specificity	The presence of total NDFIP2 can be accurately measured with the NDFIP2 antibody, which specifically detects endogenous levels of the protein. To emphasize, this antibody is designed to recognize the entire NDFIP2 protein and can effectively determine its abundance within a sample.
Species Reactivity	Human;Mouse
Antigen	NDFIP2
Application	For western blotting, the recommended dilution range is 1:500 to 1:2000. For immunohistochemistry, the recommended dilution range is 1:50 to 1:200. ELISA testing with peptide probes should be conducted using a dilution range of 1:20000 to 1:40000. It is important to adhere to these dilution recommendations to ensure accurate and reliable results.
Immunogen	A synthesized peptide.
MW	36 kDa
Properties
Concentration	1mg/ml
purification	SulfoLink™ Coupling Resin was employed in the process of purifying the antiserum through peptide affinity chromatography. The resulting product was thoroughly refined and can be considered of high quality.
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Target
Background	Activates HECT domain-containing E3 ubiquitin-protein ligases, including ITCH, NEDD4, NEDD4L, SMURF2, WWP1 and WWP2, and consequently modulates the stability of their targets. As a result, may control many cellular processes. Recruits ITCH, NEDD4 and SMURF2 to endosomal membranes. Negatively regulates KCNH2 potassium channel activity by decreasing its cell-surface expression and interfering with channel maturation through recruitment of NEDD4L to the Golgi apparatus and multivesicular body where it mediates KCNH2 degradation (PubMed:26363003). May modulate EGFR signaling. Together with NDFIP1, limits the cytokine signaling and expansion of effector Th2 T-cells by promoting degradation of JAK1, probably by ITCH- and NEDD4L-mediated ubiquitination (By similarity).
Tissue specificity	Expressed in brain, lung, heart, skeletal muscle, kidney, liver and placenta.
Posttranslational modification	Ubiquitinated by NEDD4 and ITCH. Also ubiquitinated by NEDD4L. Ubiquitination by NEDD4 or NEDD4L does not affect turnover (By similarity).Undergoes transient tyrosine-phosphorylation following EGF stimulation, most probably catalyzed by SRC. Phosphorylation on Tyr-151, Tyr-171 and Tyr-177 are dependent on the phosphorylation on Tyr-167. Also phosphorylated by LYN and FYN.
Celluar localization	Endoplasmic reticulum;Endosome;Golgi apparatus;Mitochondrion;
UniPort	Q9NV92