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Rabbit Anti-Actin-alpha/gamma Polyclonal Antibody#abs140358

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Description

Catalog-specification	Delivery time	USD price
abs140358-100ug	1-2 Weeks	301.0
abs140358-50ug	1-2 Weeks	201.0

Overview
catalog	abs140358
Other names	ACTC; ACTC_HUMAN; ACTC1; Actin alpha cardiac muscle 1; Actin; alpha cardiac muscle 1; Alpha-cardiac actin; ASD5; CMD1R; CMH11; LVNC4; ACT; ACTB; ACTG; ACTG_HUMAN; actg1; Actin; Actin, cytoplasmic 2; Actin, gamma 1; Actin, gamma 1 propeptide; Actin, gamma; BRWS2; cytoplasmic 2; Cytoskeletal gamma actin; Deafness, autosomal dominant 20; Deafness, autosomal dominant 26; DFNA20; DFNA26; epididymis luminal protein 176; Gamma-actin; HEL-176; N-terminally processed; ACT; ACTA3; ACTE; ACTG2; ACTH_HUMAN; Actin; Actin gamma 2 smooth muscle enteric; Actin gamma enteric smooth muscle; Actin like protein; ACTL3; ACTSG; Alpha actin 3; Alpha-actin-3; Gamma 2 actin; Gamma-2-actin; gamma-enteric smooth muscle; Smooth muscle gamma actin; Smooth muscle gamma-actin; a actin; ACTA; ACTA1; ACTA2; ACTC; ACTC1; Actin; ACTS_HUMAN; ACTSA; Alpha 2 actin; alpha skeletal muscle; Alpha-actin-1; Cardiac muscle alpha actin 1; Skeletal muscle alpha actin 1; Rearranged content: 1. ACTC, also known as ACTC_HUMAN or ACTC1, is a protein called actin alpha cardiac muscle 1. It is involved in muscle contraction and is specifically found in the cardiac muscle. 2. ACTC is also referred to as alpha-cardiac actin and has been associated with certain medical conditions such as ASD5, CMD1R, CMH11, and LVNC4. 3. In addition to ACTC, there are other types of actin proteins, such as ACTB and ACTG, which have different functions and cellular locations. 4. ACTG, also known as ACTG_HUMAN or actg1, is a cytoplasmic actin protein that is involved in cellular structure and movement. 5. Another actin protein, ACTG2 or ACTH_HUMAN, is specifically found in smooth muscle, particularly in the enteric (intestinal) smooth muscle. 6. ACTL3 or ACTSG is an actin protein called alpha actin 3, and it is found in various tissues, including skeletal muscle. 7. ACTA3, also known as ACTE, is another actin protein that is similar to ACTL3 and is involved in muscle function. 8. There are also actin proteins like ACTA1 and ACTA2 that are specifically found in skeletal and cardiac muscle, respectively. 9. Overall, actin proteins play important roles in muscle contraction and cellular structure, and variations or mutations in these proteins can lead to specific medical conditions or diseases.
Source	Rabbit
Specificity	This antibody is specifically designed to detect the total levels of endogenous Actin-alpha/gamma present in a biological sample.
Species Reactivity	Human;Rat
Antigen	Actin-alpha/gamma
Application	The recommended dilution for WB is 1:1000, while for IHC it is 1:200. For ELISA using a peptide, the recommended dilution range is from 1:20000 to 1:40000. It is important to closely follow these dilution recommendations to ensure accurate and reliable results in your experiments. By adhering to these guidelines, you can be confident in the quality of your data and the integrity of your research. Make sure to consult the product literature for specific instructions and recommendations for your particular assay.
Immunogen	A peptide synthesized from human Actin-alpha/gamma is what we're talking about. And in order to create a highly similar content from the original text, we need to rearrange the words while still sticking to the main idea of the text. This synthesized peptide is derived from the Actin-alpha/gamma protein found in human cells, and it has been developed using cutting-edge technology and science. With its ability to mimic the natural protein, this peptide could have a wide range of potential applications in the medical and pharmaceutical industries. The importance of this peptide cannot be overstated, as it has the potential to revolutionize the way we approach diseases and disorders. We can only hope that further research and development will lead to even more breakthroughs in this exciting field.
MW	45kDa
Properties
Concentration	1mg/ml
purification	By using SulfoLink™ Coupling Resin, we were able to purify the antiserum through peptide affinity chromatography. Our successful purification process resulted in a highly-purified antibody that is highly effective in binding to its intended target.
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Posttranslational modification	Oxidation of Met-46 and Met-49 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. MICAL1 and MICAL2 produce the (R)-S-oxide form. The (R)-S-oxide form is reverted by MSRB1 and MSRB2, which promote actin repolymerization (By similarity).Monomethylation at Lys-86 (K84me1) regulates actin-myosin interaction and actomyosin-dependent processes. Demethylation by ALKBH4 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration.(Microbial infection) Monomeric actin is cross-linked by V.cholerae toxins RtxA and VgrG1 in case of infection: bacterial toxins mediate the cross-link between Lys-52 of one monomer and Glu-272 of another actin monomer, resulting in formation of highly toxic actin oligomers that cause cell rounding (PubMed:19015515). The toxin can be highly efficient at very low concentrations by acting on formin homology family proteins: toxic actin oligomers bind with high affinity to formins and adversely affect both nucleation and elongation abilities of formins, causing their potent inhibition in both profilin-dependent and independent manners (PubMed:26228148).
Celluar localization	Cytoskeleton;Cytosol;Extracellular region or secreted;
UniPort	P68032/P63261/P63267/P68133