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Rabbit Anti-PAK1IP1 Polyclonal Antibody#abs132942

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Description

Catalog-specification	Delivery time	USD price
abs132942-100ug	1-2 Weeks	301.0
abs132942-50ug	1-2 Weeks	201.0

Overview
catalog	abs132942
Other names	FLJ20624; hPIP1; MAK11; PIP1; PAK1IP1; RP11 421M1.5; WDR84; PAK1 interacting protein; PAK- AND PLC-INTERACTING PROTEIN 1; bA421M1.5; hPIP 1; MAK 11; p21 activated protein kinase interacting protein 1; PAK/PLC interacting protein 1; Putative PAK inhibitor Skb15; WD repeat containing protein 84; WDR 84. Below is a rearranged version of the provided content: FLJ20624 is a protein known as hPIP1. It is also referred to as MAK11 and PIP1, and is associated with the interaction of PAK1, and PLC. Another name for FLJ20624 is PAK1 interacting protein 1, or PAK1IP1. It is also identified as RP11 421M1.5 and WDR84, which is a WD repeat containing protein 84. Furthermore, FLJ20624 is sometimes called PAK/PLC interacting protein 1 or Putative PAK inhibitor Skb15.
Source	Rabbit
Specificity	The PAK1IP1 antibody is capable of detecting total PAK1IP1 at endogenous levels. This means that the antibody can identify all concentrations of PAK1IP1 that naturally occur within an organism.
Species Reactivity	Human;Mouse
Antigen	PAK1IP1
Application	The dilutions for Western blot (WB) range from 1:500 to 1:2000, for immunohistochemistry (IHC) they range from 1:50 to 1:200, and for ELISA with peptide they range from 1:20000 to 1:40000. It is important to carefully consider the appropriate dilution factor for each assay to ensure accurate results.
Immunogen	A synthesized peptide.
MW	48 kDa
Properties
Concentration	1mg/ml
purification	Using SulfoLink™ Coupling Resin, we purified the antiserum through peptide affinity chromatography. Our method resulted in a highly purified product that maintained its specificity and functionality.
Clonality	Polyclonal Antibody
Stability & Storage	To maintain its quality, the product must be stored for a year at a temperature of -20 °C. It is crucial to prevent subjecting it to multiple freeze/thaw cycles.
Storage buffer	The solution of Rabbit IgG is made up of sodium chloride, glycerol, and sodium azide in phosphate buffered saline, with a pH of 7.4. The concentration of NaCl is 150mM and the solution is also fortified with 0.02% of sodium azide to preserve the contents. Additionally, the solution contains 50% glycerol for optimal preservation and storage.
Target
Background	Negatively regulates the PAK1 kinase. PAK1 is a member of the PAK kinase family, which has been shown to play a positive role in the regulation of signaling pathways involving MAPK8 and RELA. PAK1 exists as an inactive homodimer, which is activated by binding of small GTPases such as CDC42 to an N-terminal regulatory domain. PAK1IP1 also binds to the N-terminus of PAK1, and inhibits the specific activation of PAK1 by CDC42. May be involved in ribosomal large subunit assembly (PubMed:24120868).
Tissue specificity	Expressed in brain, colon, heart, kidney, liver, lung, muscle, peripheral blood leukocytes, placenta, small intestine, spleen and thymus.
Celluar localization	Nucleus;
UniPort	Q9NWT1

Western blot analysis of PAK1IP1 expression in PAK1 interacting protein 1 transfected 293T cell lysate,The lane on the left is treated with the antigen-specific peptide.

This product is for research use only, not for use in diagnostic prodecures or in human.

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