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Phospho-IRF-7 (Ser477) Rabbit Polyclonal Antibody#abs140315

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Description

Catalog-specification	Delivery time	USD price
abs140315-100ug	1-2 Weeks	301.0
abs140315-50ug	1-2 Weeks	201.0

Overview
catalog	abs140315
Other names	Interferon regulatory factor 7, also known as IRF-7 or IRF7, is a transcription factor that plays a crucial role in immune response. It is expressed in a variety of cells, including dendritic cells, B cells, and T cells. The protein's structure consists of a DNA-binding domain and an IRF association domain. IRF-7 functions as a transcriptional activator for many interferon-induced genes and is involved in the expression of type I interferons. Additionally, it is important for the induction of antiviral responses. Mutations in the IRF7 gene have been associated with susceptibility to viral infections and autoimmune disorders. Overall, IRF-7 plays a critical role in coordinating the immune response to support the body's defense against infections.
Source	Rabbit
Specificity	The IRF-7 (Phospho-Ser477) Antibody is capable of identifying the presence of IRF-7 protein in its phosphorylated form specifically at the Ser477 site. This antibody does not detect endogenous levels of IRF-7 that are not phosphorylated at Ser477.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Rabbit;Pig;Sheep;Horse;Bovine
Antigen	IRF-7
Application	WB 1:1000-3000, ELISA(peptide) 1:20000-1:40000
Immunogen	IRF-7（477），，。
MW	54kDa
Properties
Concentration	1mg/ml
purification	Sequential chromatography on phospho- and non-phospho-peptide affinity columns was utilized to purify the antibody, which was derived from rabbit serum through an affinity purification process. The resulting antibody has a high level of similarity to the original serum and is highly effective at binding to its target.
Clonality	Polyclonal Antibody
Stability & Storage	To ensure maximum stability, it is recommended to keep the product stored at a temperature of -20 °C for a period of up to one year. It is important to avoid subjecting the product to repeated cycles of freezing and thawing, as this can have a detrimental effect on its overall quality and efficacy. By following these guidelines, you can help to ensure that your product remains in the best possible condition and is ready for use whenever you need it.
Storage buffer	This product contains Rabbit IgG in 150mM NaCl and phosphate buffered saline with a pH of 7.4. It also includes 0.02% sodium azide and 50% glycerol for added stability. For optimal use, store at -20 °C and it will remain stable for 12 months from the date of receipt.
Target
Background	Key transcriptional regulator of type I interferon (IFN)-dependent immune responses and plays a critical role in the innate immune response against DNA and RNA viruses. Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. Can efficiently activate both the IFN-beta (IFNB) and the IFN-alpha (IFNA) genes and mediate their induction via both the virus-activated, MyD88-independent pathway and the TLR-activated, MyD88-dependent pathway. Required during both the early and late phases of the IFN gene induction but is more critical for the late than for the early phase. Exists in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, becomes phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization and nuclear localization where along with other coactivators it can activate transcription of the type I IFN and ISG genes. Can also play a role in regulating adaptive immune responses by inducing PSMB9/LMP2 expression, either directly or through induction of IRF1. Binds to the Q promoter (Qp) of EBV nuclear antigen 1 a (EBNA1) and may play a role in the regulation of EBV latency. Can activate distinct gene expression programs in macrophages and regulate the anti-tumor properties of primary macrophages.
Tissue specificity	Expressed predominantly in spleen, thymus and peripheral blood leukocytes.
Posttranslational modification	Acetylation inhibits its DNA-binding ability and activity.In response to a viral infection, phosphorylated on Ser-477 and Ser-479 by TBK1 and IKBKE1. Phosphorylation, and subsequent activation is inhibited by vaccinia virus protein E3. In TLR7- and TLR9-mediated signaling pathway, phosphorylated by IRAK1.TRAF6-mediated ubiquitination is required for IRF7 activation.Sumoylated by TRIM28, which inhibits its transactivation activity.
Celluar localization	Cytosol;Endosome;Nucleus;
UniPort	Q92985