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Rabbit Anti-SETDB2 Polyclonal Antibody#abs132945

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Description

Catalog-specification	Delivery time	USD price
abs132945-100ug	1-2 Weeks	301.0
abs132945-50ug	1-2 Weeks	201.0

Overview
catalog	abs132945
Description	During early development and mitosis, there is a histone methyltransferase that plays a crucial role in establishing the left-right axis. Its main function is to add three methyl groups to a specific location, called 'Lys-9', on histone H3. This modification, known as H3K9me3, is a significant epigenetic mark associated with transcriptional repression. It serves as a signal for recruiting proteins called HP1 (CBX1, CBX3, and/or CBX5) to methylated histones. By adding these methyl groups, the enzyme contributes to the establishment of H3K9me3 in repetitive elements scattered throughout the genome and specific regions near centromeres. Additionally, this enzyme is involved in the process of chromosome condensation and segregation during mitosis, ensuring the accurate distribution of genetic material.
Other names	The gene known as C13orf4, or Chromosome 13 open reading frame 4, is also referred to as CLLD8 or Chronic Lymphocytic Leukemia Deletion Region Gene 8. The protein encoded by this gene is a histone-lysine N-methyltransferase and is involved in the methylation of histone H3 at lysine 9. This gene is also known as SETDB2, which stands for SET Domain Bifurcated 2, and KMT1F, which stands for Lysine N-methyltransferase 1F. The protein coded for by C13orf4 or SETDB2 plays a key role in regulating gene expression in a range of cellular processes. This gene is located on chromosome 13 and is often deleted in individuals with chronic lymphocytic leukemia. In summary, C13orf4 is a multifunctional gene that has been implicated in various cancers and plays a crucial role in the regulation of gene expression.
Source	Rabbit
Specificity	The SETDB2 antibody is capable of identifying the overall levels of SETDB2 that exist naturally within the body. To create content that closely aligns with the information presented above, one could potentially rephrase this statement by stating that the SETDB2 antibody recognizes the endogenous levels of total SETDB2 protein.
Species Reactivity	Human;Mouse
Predictive reaction species	Zebrafish;Rabbit;Pig;Dog;Horse;Sheep;
Antigen	SETDB2
Application	The recommended dilution for Western blot is between 1:500 and 1:2000, while for immunohistochemistry it is recommended to dilute at a range of 1:50 to 1:200. When conducting ELISA assay with peptides, the suggested dilution is 1:20000 to 1:40000. It is crucial to accurately follow the recommended dilution for each assay to ensure optimal results. Diluting too much or too little can affect the sensitivity and specificity, leading to unreliable results. Therefore, it is essential to carefully consider the dilution range and adjust accordingly based on the specific experiment conditions.
Immunogen	A synthesized peptide.
MW	120 kDa
Properties
Concentration	1mg/ml
purification	The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Target
Background	Histone methyltransferase involved in left-right axis specification in early development and mitosis. Specifically trimethylates 'Lys-9' of histone H3 (H3K9me3). H3K9me3 is a specific tag for epigenetic transcriptional repression that recruits HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Contributes to H3K9me3 in both the interspersed repetitive elements and centromere-associated repeats. Plays a role in chromosome condensation and segregation during mitosis.
Tissue specificity	Ubiquitous. Highest expression in heart, testis and ovary.
Celluar localization	Cytosol;Nucleus;
UniPort	Q96T68