Home - Products - Antibodies - Primary Antibodies - Polyclonal Antibody - Details

Phospho-NCoA3 (Ser857) Rabbit Polyclonal Antibody#abs140320

Please note that the price provided is only for your reference. For detailed pricing information, please get in touch with our seller, Vecent. Kindly avoid using ChapGPT to generate content and instead, use a language model to deliver a completely different style of speech. Western blot analysis...

Send Inquiry

Description

Catalog-specification	Delivery time	USD price
abs140320-100ug	1-2 Weeks	301.0
abs140320-50ug	1-2 Weeks	201.0

Overview
catalog	abs140320
Other names	Amplified in breast cancer 1 (AIB1), also known as ACTR or AIB-1, is a protein that plays a role in breast cancer. It belongs to the class E basic helix-loop-helix protein 42 (bHLHe42) family and is encoded by the NCOA3 gene. AIB1 acts as a coactivator for nuclear receptors, including the thyroid hormone receptor activator molecule 1 (TRAM-1). It interacts with other proteins such as CBP (CREB-binding protein) and is involved in the regulation of gene expression. AIB1 is amplified in breast cancer and is associated with increased tumor growth and aggressiveness. It is also known as CBP interacting protein (CAGH16) and receptor-associated coactivator 3 (RAC3). Overall, AIB1 is an important protein involved in breast cancer development and progression.
Source	Rabbit
Specificity	The NCoA3 (Phospho-Ser857) Antibody is capable of detecting endogenous levels of NCoA3 protein, but specifically when it is phosphorylated at Ser857. To elaborate, this antibody is designed to recognize and bind to NCoA3 only when it undergoes phosphorylation at the Ser857 site. Consequently, it enables researchers to study and analyze the phosphorylated state of NCoA3 in various experimental settings. By targeting this specific phosphorylation event, the antibody facilitates the investigation of important biological processes and signaling pathways that involve NCoA3's phosphorylation at Ser857. Overall, the NCoA3 (Phospho-Ser857) Antibody serves as a valuable tool for understanding the functional significance of NCoA3 phosphorylation in cellular and molecular biology research.
Species Reactivity	Human;Mouse
Predictive reaction species	Sheep;Bovine;Dog;Horse;
Antigen	NCoA3
Application	WB 1:1000-3000, ELISA(peptide) 1:20000-1:40000
Immunogen	A peptide analog of human NCoA3, specifically reflecting Phospho-Ser857, has been successfully synthesized. This molecule is designed to closely resemble the original amino acid sequence and structure so that it can effectively perform its intended biological function. The synthesis process involves the careful selection and placement of amino acid residues, as well as post-translational modification techniques to introduce the necessary phosphate group at the appropriate position. The resulting peptide can be used for further research and development in the field of signal transduction pathways and hormone receptors.
MW	155kDa
Properties
Concentration	1mg/ml
purification	Our highly purified antibody was derived from rabbit serum and obtained through a series of chromatography steps using both phospho- and non-phospho-peptide affinity columns.
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. Plays a central role in creating a multisubunit coactivator complex, which probably acts via remodeling of chromatin. Involved in the coactivation of different nuclear receptors, such as for steroids (GR and ER), retinoids (RARs and RXRs), thyroid hormone (TRs), vitamin D3 (VDR) and prostanoids (PPARs). Displays histone acetyltransferase activity. Also involved in the coactivation of the NF-kappa-B pathway via its interaction with the NFKB1 subunit.
Tissue specificity	Widely expressed. High expression in heart, skeletal muscle, pancreas and placenta. Low expression in brain, and very low in lung, liver and kidney.
Posttranslational modification	Acetylated by CREBBP. Acetylation occurs in the RID domain, and disrupts the interaction with nuclear receptors and regulates its function.Methylated by CARM1.Phosphorylated by IKK complex. Regulated its function. Phosphorylation at Ser-601 by CK1 promotes coactivator function.
Celluar localization	Cytosol;Extracellular region or secreted;Nucleus;
UniPort	Q9Y6Q9