Phospho-PI3-kinase P85- Alpha (Tyr607) Rabbit Polyclonal Antibody#abs130868

abs130868-50ug

1-2 Weeks

201

abs130866-100ug

1-2 Weeks

301

Description

PIK3R1, a component of phosphoinositide-3-kinase, serves as a regulatory subunit with the ability to interact with certain tyrosine-phosphorylated proteins through its SH2 domain. Its primary function is to act as an adapter, facilitating the association between p110 catalytic units (found in alpha, beta, and delta enzymes) and the plasma membrane. This interaction allows p110 to phosphorylate inositol lipids. Moreover, PIK3R1 potentially participates in the regulation of the actin cytoskeleton. It plays a vital role in enabling insulin-dependent glucose uptake and glycogen synthesis in insulin-sensitive tissues. In vitro investigations have revealed that its SH2 domains engage with the phosphorylated INSR's YTHM motif. Notably, mutations in PIK3R1 can lead to severe insulin resistance. The existence of four alternatively spliced isoforms of PIK3R1 has been documented.

Other names

The gene symbol for Phosphatidylinositol 3 kinase associated p 85 alpha is GRB1 or GRB1. It is also known as p85 alpha or p85. The protein encoded by this gene is called P85A_HUMAN or Phosphatidylinositol 3 kinase regulatory subunit alpha or Phosphatidylinositol 3 kinase regulatory subunit polypeptide 1 (p85 alpha). It is also referred to as Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha or Phosphatidylinositol 3-kinase regulatory subunit alpha. Other names for this protein include Phosphoinositide 3 kinase regulatory subunit 1 (alpha) or Phosphoinositide 3 kinase regulatory subunit 1 (p85 alpha). It is also known as PI3 kinase p85 subunit alpha or PI3-kinase regulatory subunit alpha or PI3-kinase subunit p85-alpha. Additionally, it is called PI3K or PI3K regulatory subunit alpha or Pik3r1 or PtdIns 3 kinase p85 alpha or PtdIns-3-kinase regulatory subunit alpha or PtdIns-3-kinase regulatory subunit p85-alpha.

Source

Rabbit

Specificity

The Phospho-PI3-kinase p85- alpha (Tyr607) Antibody is capable of detecting the presence of PI3-kinase p85- alpha only if it has undergone phosphorylation at Tyrosine 607. This particular antibody is designed to target and locate endogenous levels of PI3-kinase p85- alpha that have been modified in this specific manner. It is important to note that this antibody will not detect PI3-kinase p85- alpha that has not undergone phosphorylation at Tyrosine 607, as this particular modification is required for recognition by the antibody. This antibody is a valuable tool for researchers studying the PI3-kinase signaling pathway and its various components.

Predictive reaction species

Rabbit;Sheep;Dog;Bovine;Horse

Application

WB (Western Blot) 1:5001:2000，IHC () 1:501:200，ICC/IF () 1:1001:500，ELISA () 1:200001:40000。

The phosphorylation site of Tyrosine 607 in human PI3-kinase p85-alpha can be utilized to synthesize a peptide. This synthesized peptide is derived from the protein sequence and designed to closely resemble the original structure.

Properties

1mg/ml

Purification

The antibody is  from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

Clonality

Polyclonal Antibody

Store at -20 °C for one year. Avoid repeated freeze/thaw cycles

Storage buffer

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Target

Background

 Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Plays an important role in signaling in response to FGFR1, FGFR2, FGFR3, FGFR4, KITLG/SCF, KIT, PDGFRA and PDGFRB. Likewise, plays a role in ITGB2 signaling (PubMed:17626883, PubMed:19805105, PubMed:7518429). Modulates the cellular response to ER stress by promoting nuclear translocation of XBP1 isoform 2 in a ER stress- and/or insulin-dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement (PubMed:20348923).

Tissue specificity

Isoform 2 is expressed in skeletal muscle and brain, and at lower levels in kidney and cardiac muscle. Isoform 2 and isoform 4 are present in skeletal muscle (at protein level).

Celluar localization

Cytosol;Endoplasmic reticulum;Nucleus;Plasma Membrane;

P27986