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Rabbit Anti-NQO2 Polyclonal Antibody#abs136854

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Description

Catalog-specification	Delivery time	USD price
abs136854-100ug	1-2 Weeks	301.0
abs136854-50ug	1-2 Weeks	201.0

Overview
catalog	abs136854
Description	NQO2, known as EC 1.10.99.2, is an important flavoprotein that plays a key role in catalyzing the 2-electron reduction of various quinones, redox dyes, and vitamin K menadione. To achieve this, NQO2 predominantly uses dihydronicotinamide riboside (NRH) as the electron donor. This process is critical for the proper functioning of the human body, and a deficiency in NQO2 can lead to various health complications. Therefore, it is crucial to understand the mechanisms of NQO2 and how it functions in the body. Thanks to groundbreaking research in this field, scientists are beginning to uncover the secrets of NQO2 and its role in human health.
Other names	NAD(P)H dehydrogenase quinone 2, also known as NQO2 or NRH dehydrogenase [quinone] 2, is an enzyme that plays a crucial role in cellular metabolism. It is encoded by the MGC94180 gene and is classified under the EC 1.10.99.2 enzyme family. NQO2 is dioxin-inducible and is involved in the oxidation-reduction reactions of various substrates. The primary function of NQO2 is to catalyze the transfer of electrons from NAD(P)H to various quinones, including menadione. This process leads to the conversion of quinones to their corresponding hydroquinones, contributing to the regulation of cellular redox balance. NQO2 is highly conserved across species and is expressed in various tissues, including the liver, kidney, and brain. The structure of NQO2 consists of a flavin adenine dinucleotide (FAD) binding domain, which is responsible for the binding and transfer of electrons, and a nucleotide binding domain. The FAD cofactor undergoes reversible reduction and oxidation during the catalytic cycle of NQO2. NQO2 has been implicated in multiple biological processes, including detoxification of xenobiotics, protection against oxidative stress, and modulation of cellular signaling pathways. Furthermore, alterations in NQO2 expression and activity have been observed in certain diseases, such as cancer, neurodegenerative disorders, and cardiovascular diseases. In summary, NQO2 is an essential enzyme involved in electron transfer reactions and cellular redox regulation. Its diverse functions highlight its importance in maintaining cellular homeostasis and its potential as a therapeutic target for various diseases.
Source	Rabbit
Specificity	The NQO2 Antibody is able to detect total NQO2 levels in the body's natural state. To produce similar content, the information in the original text can be rearranged to state that the antibody is specifically designed to recognize and bind to endogenous NQO2, allowing for accurate detection and measurement of its total levels.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Rabbit;Dog;
Antigen	NQO2
Application	The suggested dilution range for Western Blotting is 1:500 to 1:2000, whereas for Immunohistochemistry it is 1:50 to 1:200. For ELISA with peptide, the recommended dilution range is 1:20000 to 1:40000. It is important to carefully follow the dilution recommendations to achieve accurate and reliable results in your experiments.
Immunogen	A synthesized peptide derived from human NQO2.
MW	26kDa
Properties
Concentration	1mg/ml
purification	Using SulfoLink™ Coupling Resin, the antiserum was purified through the process of peptide affinity chromatography. To achieve a high degree of purity, this technique was employed which involved specific binding of peptides to the resin. The resulting product is a highly purified antiserum that can be used for various applications.
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	The enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinones involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis.
Celluar localization	Cytosol;Extracellular region or secreted;Nucleus;
UniPort	P16083