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Rabbit Anti-CDKN1A Polyclonal Antibody#abs135943

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Description

Catalog-specification	Delivery time	USD price
abs135943-100ug	1-2 Weeks	301.0
abs135943-50ug	1-2 Weeks	201.0

Overview
catalog	abs135943
Description	The protein p21 Waf1/Cip1 is a crucial tumor suppressor that controls the progression of the cell cycle. It works by forming complexes with cyclins and cyclin-dependent kinases in a stoichiometric manner and acts as an inhibitor of cell cycle progression. Specifically, when bound to CDK2 complexes, it inhibits kinase activity and thereby blocks progression through G1/S. However, p21 can also interact with CDK4 or CDK6 and cyclin D complexes, which enhances their assembly and activity. Additionally, p21 has a role in coordinating DNA replication with cell cycle progression. This is because its carboxy-terminal region can bind and inhibit PCNA, which is a subunit of DNA polymerase. Furthermore, p53, a key regulator of the cell cycle, upregulates p21 transcription in response to UV damage or during cell cycle stages when cdc2/cyclin B or CDK2/cyclin A is active. This occurs via a p53-responsive element. Finally, the protein levels of p21 are downregulated through ubiquitination and proteasomal degradation. In summary, the complex regulation of p21 plays a vital role in controlling the cell cycle and preventing the development of cancer.
Other names	CDK-interacting protein 1, also known as CDKI or Cyclin-dependent kinase inhibitor 1A (p21, Cip1), inhibits the activity of cyclin-dependent kinases and thereby acts as a potent inhibitor of cell cycle progression. This protein is encoded by the CDKN1A gene and is also known as p21 or p21Cip1/Waf1. It plays a critical role in regulating DNA synthesis and inducing cell cycle arrest in response to DNA damage or other stress stimuli. The CDK-interacting protein 1A is a potent inhibitor of cell cycle progression and is crucial for maintaining genomic integrity and preventing the development of cancer. It is encoded by the CDKN1A gene and is also referred to as p21 or p21Cip1/Waf1. This protein binds to cyclin-dependent kinases and inhibits their activity, thereby slowing down the rate of DNA synthesis and promoting cell cycle arrest. CDKI also plays a critical role in responding to various stress stimuli, including DNA damage and other cellular stresses. Its functions are crucial for maintaining cell viability under stress conditions and preventing the onset of cancer.
Source	Rabbit
Specificity	The p21 Cip1 Antibody is designed to detect the total amount of endogenous p21 Cip1 in the sample. This antibody recognizes the native form of p21 Cip1 and can provide reliable results for p21 Cip1 analysis. Make sure you accurately interpret these results to gain a better understanding of p21 Cip1 expression levels and potential functions.
Species Reactivity	Human;Mouse;Rat
Antigen	CDKN1A
Application	The recommended dilutions for WB are 1:500-1:2000, for IHC are 1:50-1:200, for IF/ICC are 1:100-1:500, and for ELISA (peptide) are 1:20000-1:40000. It is important to follow these dilutions to obtain accurate and reliable results in your experiments. Please use these dilution ranges as a guide and adjust accordingly based on your specific experimental conditions.
Immunogen	A synthesized peptide derived from human p21 Cip1.
MW	21kDa
Properties
Concentration	1mg/ml
purification	The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	May be involved in p53/TP53 mediated inhibition of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D-CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex. Inhibits DNA synthesis by DNA polymerase delta by competing with POLD3 for PCNA binding (PubMed:11595739).
Tissue specificity	Expressed in all adult tissues, with 5-fold lower levels observed in the brain.
Posttranslational modification	Phosphorylation of Thr-145 by Akt or of Ser-146 by PKC impairs binding to PCNA. Phosphorylation at Ser-114 by GSK3-beta enhances ubiquitination by the DCX(DTL) complex. Phosphorylation of Thr-145 by PIM2 enhances CDKN1A stability and inhibits cell proliferation. Phosphorylation of Thr-145 by PIM1 results in the relocation of CDKN1A to the cytoplasm and enhanced CDKN1A protein stability. UV radiation-induced phosphorylation at Thr-80 by LKB1 and at Ser-146 by NUAK1 leads to its degradation.Ubiquitinated by MKRN1; leading to polyubiquitination and 26S proteasome-dependent degradation. Ubiquitinated by the DCX(DTL) complex, also named CRL4(CDT2) complex, leading to its degradation during S phase or following UV irradiation. Ubiquitination by the DCX(DTL) complex is essential to control replication licensing and is PCNA-dependent: interacts with PCNA via its PIP-box, while the presence of the containing the 'K+4' motif in the PIP box, recruit the DCX(DTL) complex, leading to its degradation. Ubiquitination at Ser-2 leads to degradation by the proteasome pathway. Ubiquitinated by RNF114; leading to proteasomal degradation.Acetylation leads to protein stability. Acetylated in vitro on Lys-141, Lys-154, Lys-161 and Lys-163. Deacetylation by HDAC1 is prevented by competitive binding of C10orf90/FATS to HDAC1 (By similarity).
Celluar localization	Cytosol;Nucleus;
UniPort	P38936