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Rabbit Anti-CLU Polyclonal Antibody#abs135941

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Description

Catalog-specification	Delivery time	USD price
abs135941-100ug	1-2 Weeks	301.0
abs135941-50ug	1-2 Weeks	201.0

Overview
catalog	abs135941
Description	Under certain conditions of stress, the gene in question is responsible for producing a secreted chaperone protein. Interestingly, this protein can also be detected in the cell cytosol. Its involvement has been proposed in various critical biological processes including cell death, progression of tumors, and neurodegenerative disorders. Through alternate splicing, both coding and non-coding variants of the gene are generated. This information was sourced from RefSeq in May 2011. Let me know if there's anything else I can assist you with.
Other names	Clusterin, also known as apolipoprotein J, is a multifunctional glycoprotein that is involved in various physiological processes. It is encoded by the gene AAG4 or Aging Associated Protein 4 and is also known as APOJ, CLI, and SP40. Clusterin is expressed in various tissues such as the brain, liver, kidney, and testes. It exists in two forms - a secreted heterodimeric protein and a cytoplasmic monomeric protein. Clusterin has a wide range of functions such as regulating cell death, aiding in tissue repair, and modulating immune response. It is involved in the formation of extracellular matrix and acts as a chaperone protein to prevent protein aggregation. It plays a role in cell signaling pathways and is implicated in several diseases including Alzheimer's, cancer, and kidney disease. Clusterin is also known by other names such as APOJ, Complement Associated Protein SP-40, and Sugp-2. It is sometimes called by its gene symbol, AAG4, and has alternative splicing variants such as NA1/NA2 and Trpmb. It binds to Ku70 and is sometimes referred to as Ku70-binding protein 1 or KUB1. In summary, Clusterin or apolipoprotein J is a versatile protein with a broad range of functions in various biological processes. It is encoded by the gene AAG4 and is also known as APOJ, CLI, and SP40. It has alternative splicing variants such as NA1/NA2 and Trpmb and is implicated in several diseases including Alzheimer's and cancer.
Source	Rabbit
Specificity	Total CLU levels are detected by the CLU Antibody, which targets the endogenous amounts of the protein.
Species Reactivity	Human;Mouse;Rat
Antigen	CLU
Application	For Western blotting, the recommended dilution ratio ranges from 1:500 to 1:2000. When it comes to immunohistochemistry (IHC), the appropriate dilution range is 1:50 to 1:200. As for immunofluorescence/immunocytochemistry (IF/ICC), the suggested dilution ratio falls between 1:100 and 1:500. If you are performing an ELISA using a peptide, the ideal dilution range would be 1:20000 to 1:40000. Remember to adjust the dilution accordingly to achieve the best results for your experiment.
Immunogen	A synthesized peptide derived from human CLU.
MW	53kDa
Properties
Concentration	1mg/ml
purification	The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Isoform 1 functions as extracellular chaperone that prevents aggregation of nonnative proteins. Prevents stress-induced aggregation of blood plasma proteins. Inhibits formation of amyloid fibrils by APP, APOC2, B2M, CALCA, CSN3, SNCA and aggregation-prone LYZ variants (in vitro). Does not require ATP. Maintains partially unfolded proteins in a state appropriate for subsequent refolding by other chaperones, such as HSPA8/HSC70. Does not refold proteins by itself. Binding to cell surface receptors triggers internalization of the chaperone-client complex and subsequent lysosomal or proteasomal degradation. Secreted isoform 1 protects cells against apoptosis and against cytolysis by complement. Intracellular isoforms interact with ubiquitin and SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes and promote the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes proteasomal degradation of COMMD1 and IKBKB. Modulates NF-kappa-B transcriptional activity. Nuclear isoforms promote apoptosis. Mitochondrial isoforms suppress BAX-dependent release of cytochrome c into the cytoplasm and inhibit apoptosis. Plays a role in the regulation of cell proliferation.
Tissue specificity	Detected in blood plasma, cerebrospinal fluid, milk, seminal plasma and colon mucosa. Detected in the germinal center of colon lymphoid nodules and in colon parasympathetic ganglia of the Auerbach plexus (at protein level). Ubiquitous. Detected in brain, testis, ovary, liver and pancreas, and at lower levels in kidney, heart, spleen and lung.
Posttranslational modification	Isoform 1 is proteolytically cleaved on its way through the secretory system, probably within the Golgi lumen.Polyubiquitinated, leading to proteasomal degradation.Heavily N-glycosylated. About 30% of the protein mass is comprised of complex N-linked carbohydrate.
Celluar localization	Cytosol;Endoplasmic reticulum;Extracellular region or secreted;Golgi apparatus;Mitochondrion;Nucleus;
UniPort	P10909