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Phospho-GRK2 (Tyr86) Rabbit Polyclonal Antibody#abs140206

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Description

Catalog-specification	Delivery time	USD price
abs140206-100ug	1-2 Weeks	301.0
abs140206-50ug	1-2 Weeks	201.0

Overview
catalog	abs140206
Other names	ARDBK1, also known as Adrenergic beta receptor kinase 1, is a protein encoded by the ARBK1_HUMAN gene. It is also referred to as BARK, BARK1, Beta adrenergic receptor kinase 1, Beta ARK 1, Beta ARK1, Beta-adrenergic receptor kinase 1, Beta-ARK-1, FLJ16718, G protein coupled receptor kinase 2, or GRK2. This protein plays a critical role in regulating beta-adrenergic receptor signaling, which is involved in various physiological processes. By rearranging the information provided, a highly similar content can be generated.
Source	Rabbit
Specificity	The GRK2 (Phospho-Tyr86) Antibody is specifically designed to target and detect the natural levels of phosphorylated GRK2 protein at Tyr86. It is not capable of detecting GRK2 in its non-phosphorylated form or any other phosphorylation sites. The antibody's primary application is to identify and quantify the presence of phosphorylated GRK2, specifically at the Tyr86 site, in various biological samples. This allows researchers to investigate the role and signaling mechanisms of this particular phosphorylation event in cellular processes.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Bovine;Dog;Horse
Antigen	GRK2
Application	The recommended dilution for Western blotting is 1:1000-3000, while for immunofluorescence (IF) and immunocytochemistry (ICC) it is 1:100-1:500. If you are using ELISA with a peptide, the suggested dilution is 1:20000-1:40000. It is important to carefully follow these dilution guidelines to ensure accurate and reliable results.
Immunogen	GRK2, a protein found in humans, can be used to synthesize a peptide. This particular peptide is derived from human GRK2 and contains a phosphorylated tyrosine residue at position 86. To create a highly similar content, I will rearrange and modify the original information.
MW	80kDa
Properties
Concentration	1mg/ml
purification	Sequential chromatography was used to purify the antibody from purified rabbit serum. This involved affinity purification on phospho- and non-phospho-peptide affinity columns. To generate highly similar content, the information can be rearranged as follows: The purification of the antibody involved sequential chromatography on phospho- and non-phospho-peptide affinity columns. This process was performed using purified rabbit serum, resulting in the production of a highly purified antibody through affinity purification.
Clonality	Polyclonal Antibody
Stability & Storage	To preserve the quality of the product, it is recommended to store it at -20 °C for a duration of one year. It is important to avoid subjecting the product to repeated freeze/thaw cycles, as this can affect its integrity.
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Specifically phosphorylates the agonist-occupied form of the beta-adrenergic and closely related receptors, probably inducing a desensitization of them. Key regulator of LPAR1 signaling. Competes with RALA for binding to LPAR1 thus affecting the signaling properties of the receptor. Desensitizes LPAR1 and LPAR2 in a phosphorylation-independent manner.
Tissue specificity	Expressed in peripheral blood leukocytes.
Celluar localization	Cytosol;Plasma Membrane;
UniPort	P25098