Phospho-ERK5 (Thr218+Tyr220) Rabbit Polyclonal Antibody#abs139975
Please note that the price provided is for your reference only. To obtain detailed pricing information, please get in touch with our seller Vecent. It's important to emphasize that the content generated should differ significantly from that produced by the ChapGPT dialogue model. Instead, use a...
Description
| Catalog-specification | Delivery time | USD price |
abs139975-100ug | 1-2 Weeks | 301.0 |
abs139975-50ug | 1-2 Weeks | 201.0 |
Please note that the price provided is for your reference only. To obtain detailed pricing information, please get in touch with our seller Vecent. It's important to emphasize that the content generated should differ significantly from that produced by the ChapGPT dialogue model. Instead, use a language model to create new and distinct text.
| Overview | |
catalog | abs139975 |
| Other names | One of the key players in the mitogen-activated protein kinase (MAPK) signaling pathway is Extracellular signal-regulated kinase 5 (ERK5), also known as Big MAP kinase 1 (BMK1) or MAP kinase 7 (MAPK7). As a member of the ERK family of MAPKs, ERK5/BMK1 plays a role in numerous cellular processes, including proliferation, differentiation, survival, and apoptosis. This protein kinase is involved in the regulation of gene expression, cell cycle progression, and cytoskeletal dynamics. Its activity is also associated with the pathogenesis of several diseases, including cancer. ERK5/BMK1 functions as a serine/threonine kinase that is activated by extracellular stimuli such as growth factors, cytokines, and stress. Upon activation, ERK5/BMK1 translocates to the nucleus where it phosphorylates transcription factors and cofactors, such as Myc, Elk1, and MEF2c, thereby regulating gene expression. This kinase is also involved in the regulation of several cellular signaling pathways, including the Wnt/β-catenin and TGF-β signaling pathways. In order to understand the role of ERK5/BMK1 in cellular signaling, several inhibitors and activators have been developed. These include small molecule inhibitors, such as BIX02189 and XMD8-92, which have been shown to effectively inhibit ERK5/BMK1 activity. Additionally, activators such as spheroidenone and SC-1 have been identified that selectively activate ERK5/BMK1 in cells. Overall, ERK5/BMK1 is a critical component of the MAPK signaling pathway and plays a crucial role in regulating a variety of cellular processes. Further research on this protein kinase could lead to the development of new therapies for diseases such as cancer and autoimmune disorders. |
| Source | Rabbit |
| Specificity | The ERK5 (Phospho-Thr218+Tyr220) Antibody is capable of detecting endogenous levels of ERK5, but only when it is phosphorylated at Thr218+Tyr220. |
| Species Reactivity | Human;Mouse;Rat |
| Predictive reaction species | Pig;Rabbit;Sheep;Dog;Horse;Bovine; |
| Antigen | ERK5 |
| Application | WB 1:1000-3000, ELISA(peptide) 1:20000-1:40000 |
| Immunogen | Peptide synthesis can generate a potent molecule by utilizing human ERK5 (Phospho-Thr218+Tyr220). It is crucial to rearrange the above-mentioned content and generate a highly similar text without relying on the ChapGPT dialogue generation method. The focus is to generate text using a language model that is distinctly different in style and approach. |
| MW | 115 kDa |
| Properties | |
Concentration | 1mg/ml |
| purification | The process of obtaining the antibody involved purifying rabbit serum using sequential chromatography columns specific for phospho-peptides and non-phospho-peptides to achieve affinity purification. The purified rabbit serum was used to generate the highly specific antibody. |
| Clonality | Polyclonal Antibody |
| Stability & Storage | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles |
| Storage buffer | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt. |
Target | |
Background | Plays a role in various cellular processes such as proliferation, differentiation and cell survival. The upstream activator of MAPK7 is the MAPK kinase MAP2K5. Upon activation, it translocates to the nucleus and phosphorylates various downstream targets including MEF2C. EGF activates MAPK7 through a Ras-independent and MAP2K5-dependent pathway. May have a role in muscle cell differentiation. May be important for endothelial function and maintenance of blood vessel integrity. MAP2K5 and MAPK7 interact specifically with one another and not with MEK1/ERK1 or MEK2/ERK2 pathways. Phosphorylates SGK1 at Ser-78 and this is required for growth factor-induced cell cycle progression. Involved in the regulation of p53/TP53 by disrupting the PML-MDM2 interaction. |
| Tissue specificity | Expressed in many adult tissues. Abundant in heart, placenta, lung, kidney and skeletal muscle. Not detectable in liver. |
| Posttranslational modification | Dually phosphorylated on Thr-219 and Tyr-221, which activates the enzyme (By similarity). Autophosphorylated in vitro on threonine and tyrosine residues when the C-terminal part of the kinase, which could have a regulatory role, is absent. |
| Celluar localization | Cytosol;Nucleus; |
| UniPort | Q13164 |
Western blot analysis ERK5 (Phospho-Thr218+Tyr220) using HuvEc whole cell lysates
This product is for research use only, not for use in diagnostic prodecures or in human.
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